초록 ▼

A method for detecting nucleic acid sequence aberrations by detecting nucleic acid sequences having both a first and a second nucleic acid sequence type, the presence of the first and second sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration

A method for detecting nucleic acid sequence aberrations by detecting nucleic acid sequences having both a first and a second nucleic acid sequence type, the presence of the first and second sequence type on the same nucleic acid sequence indicating the presence of a nucleic acid sequence aberration. The method uses a first hybridization probe which includes a nucleic acid sequence that is complementary to a first sequence type and a first complexing agent capable of attaching to a second complexing agent and a second hybridization probe which includes a nucleic acid sequence that selectively hybridizes to the second nucleic acid sequence type over the first sequence type and includes a detectable marker for detecting the second hybridization probe.

대표청구항 ▼

[ What is claimed is:] [1.] A method for detecting nucleic acid aberrations in a sample of chromosomal DNA characterized by a rearrangement which forms a nucleic acid sequence which includes a first sequence unique to a first region of a chromosome, a second sequence unique to a second, different re

[ What is claimed is:] [1.] A method for detecting nucleic acid aberrations in a sample of chromosomal DNA characterized by a rearrangement which forms a nucleic acid sequence which includes a first sequence unique to a first region of a chromosome, a second sequence unique to a second, different region of the same chromosome or a different chromosome and a region connecting the first and second regions which includes a site of the rearrangement, the method comprising:a) contacting a first hybridization probe which includes a nucleic acid sequence complementary to the first region and a first complexing agent capable of attaching to a second complexing agent with a sample of chromosomal DNA under conditions favorable for hybridization such that the first hybridization probe hybridizes to chromosomal DNA including a nucleic acid sequence from the first region;b) contacting a second hybridization probe which includes a detectable marker and a nucleic acid sequence complementary to the second region with the sample of chromosomal DNA under conditions favorable for hybridization such that the second hybridization probe hybridizes to chromosomal DNA in the sample which include a nucleic acid sequence from the second region selectively over chromosomal DNA which include a nucleic acid sequence from the first region;c) contacting a second complexing agent attached to a solid support with the first complexing agent attached to the first hybridization probe such that the first and second complexing agents become attached, thereby immobilizing the first hybridization probe on the solid support; andd) after performing steps a-c in any order, detecting any detectable marker attached to the second hybridization probe which is immobilized on the solid support by the hybridization of the second hybridization probe to chromosomal DNA containing nucleic acid sequences from the first region which are hybridized to the first hybridization probe which is immobilized on the solid support, the first and second hybridization probes being selected such that the presence of a detectable marker immobilized on the solid support indicates the presence of a nucleic acid sequence aberration without indicating the sequence of the region connecting the first and second regions.