초록 ▼

Disclosed is a preparation of microparticles made up of a polymeric matrix and a nucleic acid expression vector. The polymeric matrix includes one or more synthetic polymers having a solubility in water of less than about 1 mg/l. At least 90% of the microparticles have a diameter less than about 100

Disclosed is a preparation of microparticles made up of a polymeric matrix and a nucleic acid expression vector. The polymeric matrix includes one or more synthetic polymers having a solubility in water of less than about 1 mg/l. At least 90% of the microparticles have a diameter less than about 100 microns. The nucleic acid is either RNA, at least 50% of which is in the form of closed circles, or circular DNA plasmid molecules, at least 50% of which are supercoiled.

대표청구항 ▼

[ What is claimed is:] [1.] A preparation of microparticles, each of which comprises a polymeric matrix and nucleic acid, the polymeric matrix consisting essentially of one or more synthetic polymers having a solubility in water of less than about 1 mg/l, whereinat least 90% of the microparticles ha

[ What is claimed is:] [1.] A preparation of microparticles, each of which comprises a polymeric matrix and nucleic acid, the polymeric matrix consisting essentially of one or more synthetic polymers having a solubility in water of less than about 1 mg/l, whereinat least 90% of the microparticles have a diameter less than about 100 microns, andthe nucleic acid is an expression vector selected from the group consisting of RNA molecules, at least 50% of which are closed circles; and circular plasmid DNA molecules, at least 50% of which are supercoiled.