초록 ▼

The present invention discloses a method for rapid antimicrobial susceptibility testing to screen antibiotics in a few hours instead of days by conventional methods. This method can also be used to identify susceptible antibiotics to treat mycobacterial infection in a few days instead of the usual s

The present invention discloses a method for rapid antimicrobial susceptibility testing to screen antibiotics in a few hours instead of days by conventional methods. This method can also be used to identify susceptible antibiotics to treat mycobacterial infection in a few days instead of the usual six to eight weeks. Fast screening of antibiotics is achieved by a short period of specimen incubation in different antibiotics embedded media to create differential bacterial counts. The differences of bacterial counts among antibiotics embedded media are subsequently amplified by DNA amplification methods for detection. Following DNA amplification, rapid quantitation and minimum inhibition concentration (MIC) determinations for a panel of antibiotics are achieved in less than one minute by fluorescence quantitation methods.

대표청구항 ▼

[ We claim:] [1.] A method for antimicrobial susceptibility testing which comprises:incubating specimens of microbe(s) or microorganism(s) in media embedded with antimicrobial agents of serial dilution concentrations for a period of time sufficient to create differential microbial or microorganism c

[ We claim:] [1.] A method for antimicrobial susceptibility testing which comprises:incubating specimens of microbe(s) or microorganism(s) in media embedded with antimicrobial agents of serial dilution concentrations for a period of time sufficient to create differential microbial or microorganism counts in test samples; amplifying the differential microbial or microorganism counts by in vitro DNA replication of DNA from the samples; adding reporter molecules to said samples, wherein said reporter molecules are specific for double stranded DNA (dsDNA), allowing complexes to form between said reporter molecule and dsDNA in samples; and observing the presence or absence of signal from the reporter molecule added to the sample, wherein said method can be completed in 12 hours.