초록 ▼

A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a substrate. In t

A method of irradiating a biological specimen with ultraviolet, in particular a polynucleotide specimen selected from DNA or RNA, or optionally a protein. In the case where the specimen is DNA or RNA, or potentially proteins, the specimen is irradiated to cross-link the specimen to a substrate. In the case where the specimen is DNA, the specimen can also be irradiated to form thymine dimers. The method uses an apparatus which permits relatively precise control of the total ultraviolet dose received by the specimen, despite any changes of ultraviolet flux from the lamps which may occur from during any one experiment, or between a number of experiments. Thus, the method allows relatively highly reproducible results to be obtained.

대표청구항 ▼

[ We claim:] [1.] A method of irradiating a polynucleotide specimen on a substrate, using an apparatus having:(a) a chamber having a repository for the substrate;(b) an ultraviolet lamp fixture disposed within the chamber, which fixture carries an ultraviolet lamp to illuminate the repository;(c) an

[ We claim:] [1.] A method of irradiating a polynucleotide specimen on a substrate, using an apparatus having:(a) a chamber having a repository for the substrate;(b) an ultraviolet lamp fixture disposed within the chamber, which fixture carries an ultraviolet lamp to illuminate the repository;(c) an energy detector which can provide an indication of the total ultraviolet radiation dose received on the repository, the energy detector having:(i) a sensor disposed in the chamber, which provides a signal corresponding to the ultraviolet flux on the repository;(ii) a detector circuit connected to the sensor, so as to provide an indication of the total ultraviolet radiation dose received on the repository based on the signal received from the sensor over time;(d) a control circuit connected to the detector circuit and the lamp fixture, which control circuit can de-energize the lamp fixture upon detector of a selected or a predetermined total ultraviolet radiation dose by the energy detector; method comprising placing the substrate carrying the polynucleotide into the chamber, then energizing the lamp fixture so that the polynucleotide specimen will be irradiated with ultraviolet radiation from the lamp until the control circuit de-energizes the lamp upon detection of the selected or predetermined total ultraviolet radiation dose.