초록

The invention concerns monoclonal antibodies which bind to the CK-MB isoenzyme but not to the B or M subunit of CK-MB or to the CK-MM and CK-BB isoenzmyes, as well as a method for the diagnostic detection of CK-MB in a homogeneous diagnostic test using these antibodies.

대표청구항 ▼

[ We claim:] [1.] A method for determining the presence or amount of CK-MB in a sample, comprising:a) contacting the sample withi) at least one first monoclonal antibody that specifically binds CK-MB, wherein said antibody is immobilized on a latex particle, andii) a further latex-bound second monoc

[ We claim:] [1.] A method for determining the presence or amount of CK-MB in a sample, comprising:a) contacting the sample withi) at least one first monoclonal antibody that specifically binds CK-MB, wherein said antibody is immobilized on a latex particle, andii) a further latex-bound second monoclonal antibody that specifically binds CK-MB, wherein said second antibody binds to a different epitope than said first antibody and does not displace the first monoclonal antibody from its binding to CK-MB, wherein said first and second monoclonal antibodies bind to said CK-MB but not to the individual B or M subunits of CK-MB and not substantially to CK-MM and CK-BB, and wherein one of the latex-bound antibodies is produced by hybridoma line DSM ACC 2058 or DSM ACC 2060, or binds to the same epitopes as antibodies produced by hybridoma line DSM ACC 2058 or DSM ACC 2060;b) measuring any increase in agglutination as a measure of the amount or presence of CK-MB in the sample.