초록 ▼

Method for in vivo introduction of a nucleic acid cassette into stem cells of intestinal epithelium. The nucleic acid cassette is introduced via vector solution. The vector solution can be delivered via the intestinal lumen in a variety of ways, including through an insertion device such as an endos

Method for in vivo introduction of a nucleic acid cassette into stem cells of intestinal epithelium. The nucleic acid cassette is introduced via vector solution. The vector solution can be delivered via the intestinal lumen in a variety of ways, including through an insertion device such as an endoscope, through catheters, through ligating and clamping the intestine after laparotomy or through slow release capsules. The vector solution once introduced into the intestinal epithelium is allowed to contact the stem cells for sufficient time for incorporation, usually between 1 and 48 hours. After sufficient incorporation, the insertion device and/or clamping and ligation procedure blockage are removed. Preferably, the procedure includes sufficient fluid to distend the intestine and provide additional access to the stem cells and the crypts. The procedure is useful in treating a variety of diseases including metabolic disorders, endocrine disorders, circulatory disorders, coagulation disorders, cancer, and gastrointestinal disease.

대표청구항 ▼

[ What we claim is:] [1.] A method for in vivo introduction of nucleic acid cassette into intestinal epithelial progenitor cells or their progeny, comprising the steps of:introducing an insertion device into a segment of the intestine;introducing a vector solution via the insertion device, said vect

[ What we claim is:] [1.] A method for in vivo introduction of nucleic acid cassette into intestinal epithelial progenitor cells or their progeny, comprising the steps of:introducing an insertion device into a segment of the intestine;introducing a vector solution via the insertion device, said vector solution containing the nucleic acid cassette;contacting said cells or their progeny with the vector solution for sufficient time to incorporate the nucleic acid cassette into said cells or their progeny; andremoving the insertion device.