초록 ▼

A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear

A method for Agrobacterium-mediated transformation and regeneration of soybean is disclosed. The method utilizes a cotyledon explant which is prepared by first removing the hypocotyl and then tearing the two cotyledons apart at the cotyledonary node. The explant may be inoculated with either a smear of the disarmed Agrobacterium vector or of liquid culture of the bacterium.

대표청구항 ▼

[ We claim:] [1.] A method for transforming soybean which comprises:(a) preparing a cotyledon explant from a soybean seedling by:(i) removing the hypocotyl region by cutting just below the cotyledonary node,(ii) separating the two cotyledons at the cotyledonary node by tearing the cotyledons apart,

[ We claim:] [1.] A method for transforming soybean which comprises:(a) preparing a cotyledon explant from a soybean seedling by:(i) removing the hypocotyl region by cutting just below the cotyledonary node,(ii) separating the two cotyledons at the cotyledonary node by tearing the cotyledons apart, and(iii) removing the epicotyl from the cotyledon to which it remains attached,(b) inserting a chimeric gene into the explant of part (a) by inoculation and co-cultivation of the explant with a disarmed Agrobacterium tumefaciens vector containing said chimeric gene;(c) selecting transformed explant tissue, and(d) regenerating a differentiated transformed plant from the transformed explant tissue of part (c).