초록 ▼

The invention provides a method of detecting a class of substances, by providing an assay configuration which allows for reaction with a homologous portion common to all members of the class. The configuration utilizes a capture reactant to immobilize class members, revealing previously unrecognizab

The invention provides a method of detecting a class of substances, by providing an assay configuration which allows for reaction with a homologous portion common to all members of the class. The configuration utilizes a capture reactant to immobilize class members, revealing previously unrecognizable reaction sites on the class. The capture reactant interacts with a homologous portion on the class members. The immobilized class is then detected, such as by the use of a sandwich antibody directed to another homologous portion of the class now exposed for reaction, or directed to specific portions of specific members of the class that have been exposed for reaction. These reaction sites were unrecognizable prior to immobilization of the class members with the capture reactant.

대표청구항 ▼

[ What is claimed:] [1.] An immunoassay for detecting members of a class of molecules in a sample, each member of the class having a homologous portion common to members of the class, each member of the class being from a different species, said method comprising:a. obtaining a panel of antibodies g

[ What is claimed:] [1.] An immunoassay for detecting members of a class of molecules in a sample, each member of the class having a homologous portion common to members of the class, each member of the class being from a different species, said method comprising:a. obtaining a panel of antibodies generated to recognize and bind a plurality of members of the class, and which were not generated to recognize and bind the member included in the sample;b. selecting an antibody from (a) having a high binding affinity for the member at the class included in the samplec. attaching the antibody selected in (b) to an immunological reaction surface;d. adding the sample to the immunological reaction surface under conditions so that the attached antibody recognizes and binds a member of the class so as to form a complex, the attached antibody reacting with and binding the homologous portion of the class; ande. detecting the complex so bound thereby detecting members of a class.