초록 ▼

A method of identifying peptides which mimic biologically active proteins is disclosed. The method comprises the steps of making a recombinant antibody library from genetic material obtained from an animal which has been immunized against antibodies that bind to the biological active protein to the

A method of identifying peptides which mimic biologically active proteins is disclosed. The method comprises the steps of making a recombinant antibody library from genetic material obtained from an animal which has been immunized against antibodies that bind to the biological active protein to the mimicked. Recombinant antibodies are screened to identify antibodies which compete with the biological active protein. Peptides which comprise the recombinant antibody's CDR sequences are synthesized. Synthetic peptides which mimic GM-CSF are also disclosed.

대표청구항 ▼

[ We claim:] [1.] A method of identifying a peptide having an amino acid sequence consisting of 15-25 amino acids, wherein said peptide mimics binding activity of a helix of an active region of a biologically active protein to its receptor in which an active region of said biologically active protei

[ We claim:] [1.] A method of identifying a peptide having an amino acid sequence consisting of 15-25 amino acids, wherein said peptide mimics binding activity of a helix of an active region of a biologically active protein to its receptor in which an active region of said biologically active protein is a helix, said method comprising the steps of:a) inoculating a first animal with an amount of a biologically active protein sufficient to invoke an immune response in said first animal which includes production of anti-biologically active protein antibodies by said first animal, wherein an active region of said biologically active protein is a helix;b) isolating said anti-biologically active protein antibodies from said first animal;c) inoculating a second animal with an amount of the isolated anti-biologically active protein antibodies sufficient to invoke an immune response which includes production by said second animal of antibodies which specifically bind to anti-biologically active protein antibodies;d) isolating RNA from spleen cells from said second animal;e) generating cDNA from said RNA;f) amplifying fragments of said cDNA that encode antibody light chain regions including a complementarity determining region, and inserting said fragments into expression vectors to form recombinant expression vectors, wherein said expression vectors provide signal sequences to cDNA fragments for secretion of proteins encoded thereby;g) transforming suitable host cells with said recombinant expression vectors to produce transformed host cells;h) maintaining said transformed host cells under conditions which allow for expression of said fragments to produce proteins encoded thereby, wherein said proteins are secreted;i) screening said proteins to identify a recombinant antibody light chain which competes with said biological active protein to bind with a neutralizing anti-biologically active protein monoclonal antibody;j) identifying the amino acid sequences of complementarity determining regions of said recombinant antibody light chain which competes with said helix of an active region of a biological active protein to bind with said neutralizing anti-biologically active protein monoclonal antibody andk) synthesizing a peptide with an amino acid sequence that consists of between 15-25 amino acid residues and which comprises an amino acid sequence of a complementarity determining region of said recombinant antibody wherein said peptide mimics the binding activity of a helix of an active region of a biologically active protein to its receptor.