초록 ▼

The invention provides devices and methods for use in detecting nucleic acid analytes in samples. The devices each include a solid support to which is bound a two-dimensional distribution or field of nucleic acid probes that each bind to a nucleic acid analyte, which is detected by use of amplificat

The invention provides devices and methods for use in detecting nucleic acid analytes in samples. The devices each include a solid support to which is bound a two-dimensional distribution or field of nucleic acid probes that each bind to a nucleic acid analyte, which is detected by use of amplification methods.

대표청구항 ▼

[ What is claimed is:] [5.] A method for detecting the presence of a nucleic acid analyte in a sample, said method comprising the steps of:(a) applying the sample to a solid support having covalently bound thereto a two-dimensional field of first analyte-specific nucleic acid probes, each of the fir

[ What is claimed is:] [5.] A method for detecting the presence of a nucleic acid analyte in a sample, said method comprising the steps of:(a) applying the sample to a solid support having covalently bound thereto a two-dimensional field of first analyte-specific nucleic acid probes, each of the first probes comprising a first end bound to the solid support and including a 5' portion of an autocatalytically replicable nucleic acid, and a second end including a first analyte-binding segment, wherein the first analyte-binding segment of each of the first probes hybridizes to a first region of the analyte;(b) applying a second nucleic acid probe to the solid support, the second probe comprising a second analyte-binding segment that hybridizes to a second region of the analyte, the second probe further comprising a remainder of said autocatalytically replicable nucleic acid, wherein the first and second regions of the analyte are adjacent nucleotide segments, and wherein the analyte, the first probe, and the second probe hybridize together on the support to form a complex including a complete autocatalytically replicable nucleic acid;(c) applying a diffusion limiting matrix to the solid support;(d) amplifying the complete autocatalytically replicable nucleic acid to generate an amplified product; and(e) detecting the amplified product as a measure of the presence of the analyte in the sample.