초록 ▼

DNA chips containing arrays of oligonucleotide probes can be used to determine whether a target nucleic acid has a nucleotide sequence identical to or different from a specific reference sequence. The array of probes comprises probes exactly complementary to the reference sequence, as well as probes

DNA chips containing arrays of oligonucleotide probes can be used to determine whether a target nucleic acid has a nucleotide sequence identical to or different from a specific reference sequence. The array of probes comprises probes exactly complementary to the reference sequence, as well as probes that differ by one or more bases from the exactly complementary probes.

대표청구항 ▼

[ We claim:] [1.] An array of oligonucleotide probes immobilized on a solid support, said array having at least 100 probes and no more than 100,000 different oligonucleotide probes 9 to 20 nucleotides in length occupying separate known sites in said array, said oligonucleotide probes comprising at l

[ We claim:] [1.] An array of oligonucleotide probes immobilized on a solid support, said array having at least 100 probes and no more than 100,000 different oligonucleotide probes 9 to 20 nucleotides in length occupying separate known sites in said array, said oligonucleotide probes comprising at least four sets of probes: (1) a first set that is exactly complementary to a reference sequence and comprises probes that completely span the reference sequence and, relative to the reference sequence, overlap one another in sequence; and (2) three additional sets of probes, each of which is identical to said first set of probes but for at least one different nucleotide, which different nucleotide is located in the same position in each of the three additional sets but which is a different nucleotide in each set.