[특허]Light scattering optical waveguide method for detecting specific binding events

Light scattering optical waveguide method for detecting specific binding events 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	C12Q-001/68 C12Q-001/70 C07H-021/04 G01N-025/20
출원번호	US-0434354 (1995-05-02)
발명자 / 주소	Stimpson Donald Irvine Gordon Julian Hoijer Joanell V.
출원인 / 주소	Abbott Laboratories
대리인 / 주소	Brainard
인용정보	피인용 횟수 : 75 인용 특허 : 0

초록 ▼

A waveguide binding assay method involves detecting the scattering of light directed into the waveguide, the scattering being the result of scattering labels specifically bound to the waveguide within the penetration depth of an evanescent wave. The waveguide may be transparent plastic or glass and the binding is typically by oligonucleotide hybridization or immunological capture. Light scattering labels include colloidal metals or non-metals, including gold, selenium and latex. A light absorbing member consisting of dye or concentrated particles may also be employed to enhance signal. Real-time binding and dissociation can be monitored visually or by video imaging, such as with a CCD camera and frame grabber software. Hybridization mismatches of as few as one base can be distinguished by real-time melting curves.

대표청구항 ▼

[ We claim:] [1.] A method for determining base differences between at least two nucleic acid sequences, the method comprising:(a) providing a waveguide device, the waveguide device comprising (i) a transparent element having a refractive index greater than that of the fluid sample; (ii) a light receiving edge; and (iii) a reactive surface comprising a plurality of sites having oligonucleotide immobilized thereon, said sites defining an array of oligonucleotides, said reactive surface further comprising other non-situs portions having no oligonucleotides immobilized thereon;(b) contacting the reactive surface under hybridizing conditions with said at least two nucleic acid sequences wherein said nucleic acid sequences, either directly or through intermediate cognate binding pairs, is labeled with a light scattering label; thereby forming light scattering label complexes attached at those sites of the reactive surface having an oligonucleotide complementary to said nucleic acid sequences immobilized thereon;(c) illuminating the light receiving edge of the waveguide with light effective to create total internal reflection within the waveguide, thereby simultaneously illuminating the entire reactive surface;(d) collecting light scattered across the entire reactive surface by said light scattering label;(e) comparing the degree of light scattering at each situs with either (i) the degree of light scattering at a non-situs portion; or (ii) the degree of light scattering at another situs;(f) incrementally increasing the stringency conditions at the reactive surface of the waveguide device to initiate dissociation of bound nucleic acid from the sites wherein said comparing of step (e) is continued during the incremental stringency increase;whereby base differences between the at least two oligonucleotides can be distinguished by differences in dissociation properties.

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IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

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연합인증

Light scattering optical waveguide method for detecting specific binding events 원문보기

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Light scattering optical waveguide method for detecting specific binding events 원문보기

초록 ▼

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연구과제 타임라인

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