초록 ▼

A system for performing molecular biological diagnosis, analysis and multistep and multiplex reactions utilizes a selfaddressable, selfassembling microelectronic system for actively carrying out controlled reactions in microscopic formats. Preferably, a fluidic system flow a sample across an active

A system for performing molecular biological diagnosis, analysis and multistep and multiplex reactions utilizes a selfaddressable, selfassembling microelectronic system for actively carrying out controlled reactions in microscopic formats. Preferably, a fluidic system flow a sample across an active area of the biochip, increasing diagnostic efficiency. Preferably, the fluidic system includes aflow cell having a window. Pulsed activation of the electrodes of the biochip are advantageously employed with the fluidic system, permitting more complete sampling of the materials within the biological sample. An improved detection system utilizes a preferably coaxially oriented excitation fiber, such as a fiber optic, disposed within a light guide, such as a liquid light guide. In this way, small geometry systems may be fluorescently imaged. A highly automated DNA diagnostic system results. Perturbation of the fluorescence signal during electronic denaturation is detailed and analyzed for analytical and diagnostic purposes. Such fluorescence perturbation information is combined with other information to provide improved analysis. DNA fingerprinting uses hybridizing DNA fragments of a given length and a capture sequence at a test site and then determining the level of reverse bias necessary to affect the hybridization, such as to dehybridize, and determine the length of the DNA.

대표청구항 ▼

[ We claim:] [1.] A method for hybridization analysis between a sample and a probe, the analysis utilizing an electronic stringency control device, comprising the steps of:providing the sample and probe with a fluorescent label under hybridization conditions on the electronic stringency control devi

[ We claim:] [1.] A method for hybridization analysis between a sample and a probe, the analysis utilizing an electronic stringency control device, comprising the steps of:providing the sample and probe with a fluorescent label under hybridization conditions on the electronic stringency control device, forming a fluorescently labelled hybridization product,monitoring the fluorescence from the hybridization product,subjecting the hybridization product to varying electrophoretic force, andanalyzing the fluorescent signal from the flourescently labeled hybridization product for variance as a function of said varying electrophoretic force.