초록 ▼

The present invention is directed to a method and a formulation for the stabilization of an enzyme for storage at temperatures above freezing. Specifically, the invention involves the use of formulated excipients that when added to an enzyme or mixture of enzymes and subsequently lyophilized renders

The present invention is directed to a method and a formulation for the stabilization of an enzyme for storage at temperatures above freezing. Specifically, the invention involves the use of formulated excipients that when added to an enzyme or mixture of enzymes and subsequently lyophilized renders stability. Upon reconstitution, the stabilized materials are useful in assays, diagnostic or molecular biology research kits, and other biological applications.

대표청구항 ▼

[ What is claimed is:] [1.] A method for stabilizing one or more enzymes, or enzymatically active fragments thereof, for storage in the dry phase above freezing temperature, wherein said one or more enzymes is selected from the group consisting of T7 RNA polymerase, AMV-Reverse Transcriptase, RNase

[ What is claimed is:] [1.] A method for stabilizing one or more enzymes, or enzymatically active fragments thereof, for storage in the dry phase above freezing temperature, wherein said one or more enzymes is selected from the group consisting of T7 RNA polymerase, AMV-Reverse Transcriptase, RNase H, and combinations thereof, the method comprising:(i) combining said one or more enzymes or enzymatically active fragment thereof with an aqueous stabilizing solution comprising in combination a carrier protein, from about 3% to about 15% w/v of one or more disaccharides, from about 1% to about 10% w/v of one or more disaccharide derivatives, from 0% to about 10% w/v of one or more sugar polymers, and a compatible buffer thereby producing a mixture;(ii) freezing the mixture obtained in step (i); and(iii) lyophilizing the frozen mixture obtained in step (ii) until substantially water-free, whereby a stabilized enzyme formulation is obtained.