최소 단어 이상 선택하여야 합니다.
최대 10 단어까지만 선택 가능합니다.
다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
NTIS 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
DataON 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Edison 바로가기다음과 같은 기능을 한번의 로그인으로 사용 할 수 있습니다.
Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0040430 (1993-03-31) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 71 인용 특허 : 356 |
A chromatographic assay device for use with immunoassays allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from particu
A chromatographic assay device for use with immunoassays allows rapid and convenient assays of analytes of biological interest, and permits extractions to be carried out in situ, avoiding the use of separate extraction vessels. The device has a wide dynamic range and avoids interference from particulates or colored components. In one form, the device comprises: (1) a first opposable component comprising a sample preparation zone adapted to receive a sample to be assayed; and (2) a second opposable component comprising a chromatographic medium. The first and second opposable components can be brought into opposition so as to cause the sample preparation zone to apply the sample to be tested to the chromatographic medium. Preferably, the analyte is detected with a visually detectable label. Other variations of the device vary the arrangement of components to provide optimal chromatography for a variety of analytes, as well as to permit bidirectional chromatography; still other variations are suitable for competitive immunoassays. The devices can be incorporated in test kits, and assay methods, both sandwich and competitive, using the devices are also disclosed.
[ We claim:] [1.] A chromatographic assay device for detection or determination of an analyte in a sample comprising:(a) a first opposable component including:(i) a chromatographic medium having first and second ends and a detection zone, the chromatographic medium having a reagent bound at the dete
[ We claim:] [1.] A chromatographic assay device for detection or determination of an analyte in a sample comprising:(a) a first opposable component including:(i) a chromatographic medium having first and second ends and a detection zone, the chromatographic medium having a reagent bound at the detection zone, the reagent binding specifically to an analyte to be detected or determined;(ii) a detector application pad either containing a mobile reagent for detection or determination of the analyte, the mobile reagent to be subsequently applied to the chromatographic medium during the performance of an assay or for application of the mobile reagent to the pad during the performance of the assay, in operable contact with the first end of the chromatographic medium;(iii) a conductor for allowing the passage of fluid in operable contact with the detector application pad and in direct contact with the first end of the chromatographic medium; and(iv) an absorber for absorbing fluid in operable contact with the second end of the chromatographic medium; and(b) a second opposable component including a sample preparation zone for receiving a sample, the second opposable component being attachable to the first opposable component so that the first and second opposable components are brought into opposition from a position in which they are not in opposition and fluid is transferred from the second opposable component to the first opposable component by pressure; rein the first and second opposable components are configured so that a sample can be applied to the sample preparation zone when the first and second opposable components are not in opposition and bringing the first and second opposable components into opposition results in the sample preparation zone being in contact with the conductor to apply the sample to be tested to the conductor for flow through the conductor and then to the first end of the chromatographic medium through the detector application pad to add the reagent for detection of the analyte to the sample, the fluid flow being aided by absorption of fluid from the absorber, so that the analyte is detected or determined within the chromatographic medium at the detection zone after migration by binding of the reagent for detection of the analyte to the analyte bound to the detection zone, the assay being either a sandwich assay or a competitive assay and the reagent for detection of the analyte being an analyte analog for a competitive assay and a labeled primary or second antibody for a sandwich assay.
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