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Transgenic swine compositions and methods

특허상세정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) C12N-005/00    C12N-015/00   
미국특허분류(USC) 435/325 ; 435/172.3 ; 435/378
출원번호 US-0473030 (1995-06-06)
발명자 / 주소
출원인 / 주소
대리인 / 주소
    Brinks Hofer Gilson & Lione
인용정보 피인용 횟수 : 134  인용 특허 : 5
초록
▼

Transgenic swine, and compositions and methods for making and using same, are provided. Central to the invention are porcine (Sus scrofa) embryonic stem cell lines and methods for establishing them. Cells of such lines are transformed with exogenous genetic material of interest and then used to provide chimeric swine, which have germ cells comprising the exogenous genetic material. The chimeric swine are bred to provide transgenic swine. Transgenic swine of the invention can be used to provide human proteins or peptide hormones or can be used as xenograf...

대표
청구항
▼

[ What is claimed is:] [1.] A method of isolating and purifying an embryonic stem cell culture, said method comprising:(a) culturing dissociated cells from a swine embryo in conditioned stem cell medium in the absence of a feeder layer, wherein the dissociated cells are obtained from a swine embryo which was developed in vitro in stem cell medium (SCM) on a feeder layer; and(b) subculturing the culture until a stable culture with morphological features and growth parameters characteristic of an embryonic stem cell culture is established. [4.] An isolated...

이 특허에 인용된 특허 (5)

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  5. Wagner Thomas E. (Athens OH) Chen Xiao-Zhuo (Athens OH). Virus-resistant transgenic mice. USP1992125175385.

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