초록 ▼

Solutions and methods are disclosed for the effective, simple isolation/extraction of DNA, RNA and proteins from a single biological material sample, such as cells, tissues and biological fluids. The preferred solutions include effective amounts of a chaotropic agent(s), buffer, reducing agent, and

Solutions and methods are disclosed for the effective, simple isolation/extraction of DNA, RNA and proteins from a single biological material sample, such as cells, tissues and biological fluids. The preferred solutions include effective amounts of a chaotropic agent(s), buffer, reducing agent, and may or may not include an organic solvent. Genomic DNA and total RNA can be isolated utilizing the solutions and methods of the invention in as little as 20 minutes, and proteins in as little as 30 minutes.

대표청구항 ▼

[ What is claimed is:] [1.] A solution for isolating substantially pure and undegraded RNA, DNA and proteins from biological material, said solution comprising:at least one chaotropic agent,a buffer present in an amount sufficient to maintain the pH of said solution in the range of about 6 to about

[ What is claimed is:] [1.] A solution for isolating substantially pure and undegraded RNA, DNA and proteins from biological material, said solution comprising:at least one chaotropic agent,a buffer present in an amount sufficient to maintain the pH of said solution in the range of about 6 to about 7.5.an organic solvent present at a concentration in the range of about 13 to about 23% (v/v) of said solution, andat least one chelating agent.