초록 ▼

The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non-polar separatio

The invention recognizes the deleterious effects of trace, and even undetectable amounts of multivalent cations on the separation of mixtures of polynucleotides, especially double stranded polynucleotides, and provides an improved method for separating such mixtures on wide pore, non-polar separation media by eliminating multivalent cations from the all aspects of the separation process. This is accomplished by using components in the separation process which are materials which do not release metal cations. In addition, the use of cation capture resins and other methods to remove residual traces of multivalent cations from eluting solvents, sample solutions, separation media, and system components is described. It is also important to remove any traces or organic contaminants from solvents solutions and system parts. Taking similar steps to remove residual traces of multivalent cations and organic impurities from the separation process, the invention may also be used in a batch process to separate mixtures of polynucleotide fragments.

대표청구항 ▼

[ What is claimed is:] [1.] A method for separating a mixture of polynucleotide fragments comprisinga) applying a solution of said fragments and counterion reagent to a column containing, separation media having a non-polar surface, wherein said separation media have a pore size greater than 30 Angs

[ What is claimed is:] [1.] A method for separating a mixture of polynucleotide fragments comprisinga) applying a solution of said fragments and counterion reagent to a column containing, separation media having a non-polar surface, wherein said separation media have a pore size greater than 30 Angstroms and an average diameter of 1-100 microns;b) eluting said fragments with a gradient eluting solvent of increasing organic component concentration containing a counterion agent; rein surfaces which are contacted by the solution of the fragments and the eluting solvent are materials which do not trap or release multivalent metal cations therefrom.