$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

Low pH RNA isolation reagents, method, and kit 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • C07H-021/00
출원번호 US-0867243 (1997-06-02)
발명자 / 주소
  • Heath Ellen M.
출원인 / 주소
  • Gentra Systems, Inc.
대리인 / 주소
    Dorsey & Whitney LLPGlover
인용정보 피인용 횟수 : 46  인용 특허 : 7

초록 ▼

The present invention describes an RNA isolation process which utilizes low pH reagents. In addition, the reagents are less hazardous and are more stable than those used in prior art methods. This rapid method may be used to obtain purified RNA from a variety of biological sources including human wh

대표청구항 ▼

[ What is claimed is:] [1.] A method for isolating RNA from a biological sample comprising:(a) contacting the biological sample with a Cell Lysis Reagent to form a lysate containing undegraded RNA; where the Cell Lysis Reagent consists essentially of:(i) an anionic detergent;(ii) a chelating agent;(

이 특허에 인용된 특허 (7)

  1. Bernhard Susan L. (Menlo Park CA) Better Marc D. (Los Angeles CA) Carroll Steve F. (Walnut Creek CA) Lane Julie A. (Castro Valley CA) Lei Shau-Ping (Los Angeles CA), Materials comprising and methods of preparation and use for ribosome-inactivating proteins.
    상세보기
  2. Beckage Cheryl A. (Indianapolis IN) Ingolia Thomas D. (Indianapolis IN), Method, vectors and transformants for high expression of heterologous polypeptides in yeast.
    상세보기
  3. Koller Charles A. (Houston TX), Methods for isolation of nucleic acids from eukaryotic and prokaryotic sources.
    상세보기
  4. Ness Jeffrey V. (Bothell WA) Cimler B. Melina (Portland OR) Meyer ; Jr. Rich B. (Woodinville WA) Vermeulen Nicolaas M. J. (Woodinville WA), Non toxic compositions and methods useful for the extraction of nucleic acids.
    상세보기
  5. Macfarlane Donald E. (943 Iowa Ave. Iowa City IA 52240), Process for purifying DNA and RNA using cationic detergents.
    상세보기
  6. Chomczynski Piotr (727 Martin Luther King Dr. Cincinnati OH 45220), Product and process for isolating RNA.
    상세보기
  7. Burgoyne Leigh A. (Seaford AUX), Solid medium and method for DNA storage.
    상세보기

이 특허를 인용한 특허 (46)

  1. McGlennen, Ronald C.; Williamson, Naomi M.; Franks, Aaron M.; Olson, David J.; Schuldt, Robert P.; Matthias-Hagen, Vickie, Apparatus and methods for medical testing.
    상세보기
  2. Heath, Ellen M.; Shuman, Ruth M., Apparatuses and methods for isolating nucleic acid.
    상세보기
  3. Engel,Laurie; Schultz,John W.; Johnson,Tonny M.; Zimmerman,Kristopher; Bozek,Laura L.; Stevens,Judith N., Cell lysis composition, methods of use, apparatus and kit.
    상세보기
  4. Bair, Robert Jackson; Benedict, Kristen Campbell; Kivens, Wendy J.; Kwiatkowski, Jr., Robert W.; Paulsen, Kim; Strom, Daniel A.; Wages, John M., Compositions and methods for using a solid support to purify DNA.
    상세보기
  5. Bair, Jr., Robert Jackson; Heath, Ellen M.; Meehan, Heather; Paulsen, Kim Elayne; Wages, Jr., John M., Compositions and methods for using a solid support to purify RNA.
    상세보기
  6. Bair, Jr.,Robert Jackson; Heath,Ellen M.; Meehan,Heather; Paulsen,Kim Elayne; Wages, Jr.,John M., Compositions and methods for using a solid support to purify RNA.
    상세보기
  7. Bost, Douglas A.; Greenfield, Lawrence, Compositions and methods of selective nucleic acid isolation.
    상세보기
  8. Bost, Douglas A.; Greenfield, Lawrence, Compositions and methods of selective nucleic acid isolation.
    상세보기
  9. Bost, Douglas; Greenfield, Lawrence, Compositions and methods of selective nucleic acid isolation.
    상세보기
  10. Bost,Douglas A.; Greenfield,Lawrence, Compositions and methods of selective nucleic acid isolation.
    상세보기
  11. Pasloske, Brittan L.; Fang, Xingwang; Hoang, Quoc, Crude biological derivatives competent for nucleic acid detection.
    상세보기
  12. Kreader, Carol Ann; Backus, John Wesley, Electrophoretic separation of nucleic acids from proteins at low ph.
    상세보기
  13. Heath, Ellen M.; Shuman, Ruth M., Eluting reagents, methods and kits for isolating DNA.
    상세보기
  14. Hillebrand, Timo; Bendzko, Peter, Formulations and method for isolating nucleic acids from optional complex starting material and subsequent complex gene analytics.
    상세보기
  15. Paulsen,Kim E., Formulations and methods for denaturing proteins.
    상세보기
  16. Wohlgemuth, Jay; Tayama, Darren; Walther, Dirk; Lal, Preeti G., Genes useful for diagnosing and monitoring inflammation related disorders.
    상세보기
  17. Deka, Chiranjit, Light scatter based apparatus and methods for hematology analysis using only three detectors.
    상세보기
  18. Douglas T. Gjerde ; David P. Hornby GB; Christopher P. Hanna ; Alexander I. Kuklin, Method and system for RNA analysis by matched ion polynucleotide chromatography.
    상세보기
  19. Gjerde, Douglas T.; Hornby, David P.; Hanna, Christopher P.; Kuklin, Alexander I.; Haefele, Robert M.; Taylor, Paul D., Method and system for RNA analysis by matched ion polynucleotide chromatography.
    상세보기
  20. Hecker, Karl H.; Azarani, Arezou; Hornby, David; Hanna, Christopher P.; Gjerde, Douglas T.; Matin, Maryam, Method and system for the preparation of cDNA.
    상세보기
  21. Christoffel, Gabriele, Method of isolating purified RNA with reduced DNA contaminations.
    상세보기
  22. Heath, Ellen M.; Morken, Nathaniel W.; Benedict, Kristen Campbell, Method to isolate DNA.
    상세보기
  23. Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing and monitoring auto immune and chronic inflammatory diseases.
    상세보기
  24. Rosenberg, Steven; Fry, Kirk; Wu, Bin; Dedrick, Russell L., Methods and compositions for diagnosing and monitoring the status of transplant rejection and immune disorders.
    상세보기
  25. Wohlgemuth, Jay; Fry, Kirk E.; Woodward, Robert; Ly, Ngoc; Prentice, James; Morris, Macdonald; Rosenberg, Steven, Methods and compositions for diagnosing and monitoring transplant rejection.
    상세보기
  26. Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing and monitoring transplant rejection.
    상세보기
  27. Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc; Prentice, James; Morris, MacDonald; Rosenberg, Steven, Methods and compositions for diagnosing and monitoring transplant rejection.
    상세보기
  28. Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc; Prentice, James; Morris, MacDonald; Rosenberg, Steven, Methods and compositions for diagnosing and monitoring transplant rejection.
    상세보기
  29. Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc; Prentice, James; Morris, MacDonald; Rosenberg, Steven, Methods and compositions for diagnosing and monitoring transplant rejection.
    상세보기
  30. Wohlgemuth,Jay; Fry,Kirk; Woodward,Robert; Ly,Ngoc, Methods and compositions for diagnosing and monitoring transplant rejection.
    상세보기
  31. Wohlgemuth,Jay; Fry,Kirk; Woodward,Robert; Ly,Ngoc; Prentice,James, Methods and compositions for diagnosing and monitoring transplant rejection.
    상세보기
  32. Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases.
    상세보기
  33. Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing or monitoring autoimmune and chronic inflammatory diseases.
    상세보기
  34. Wohlgemuth, Jay; Fry, Kirk; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosing or monitoring autoimmune and chronic inflammatory diseases.
    상세보기
  35. Wohlgemuth, Jay; Fry, Kirk E.; Woodward, Robert; Ly, Ngoc, Methods and compositions for diagnosis and monitoring auto immune and chronic inflammatory diseases.
    상세보기
  36. Lal, Preeti G.; Williams, Gavin E.; Fry, Kirk E.; Sun, Jingtao; Dedrick, Russell L., Methods for diagnosing and monitoring the status of systemic lupus erythematosus.
    상세보기
  37. Gjerde, Douglas T.; Taylor, Paul D., Non-polar media for polynucleotide separations.
    상세보기
  38. Kudlicki,W. Antoni; Winkler,Matthew M.; Pasloske,Brittan L., Nuclease inhibitor cocktail.
    상세보기
  39. Kudlicki,W. Antoni; Winkler,Matthew M.; Pasloske,Brittan L., Nuclease inhibitor cocktail.
    상세보기
  40. Latham,Gary J.; Winkler,Matthew M.; Pasloske,Brittan L.; Kudlicki,W. Antoni, Nuclease inhibitor cocktail.
    상세보기
  41. Gundling, Gerard, Nucleic acid isolation method and kit.
    상세보기
  42. Belly,Robert; Toner,Jacqueline; Backus,John, Rapid extraction of RNA from cells and tissues.
    상세보기
  43. Chomczynski, Piotr, Reagents and methods for isolation of purified RNA.
    상세보기
  44. Chomczynski, Piotr, Reagents for isolation of purified RNA.
    상세보기
  45. Chelliserry, Jijumon; Gao, Kui; Linnen, Jeffrey M., Red blood cell lysis solution.
    상세보기
  46. Baker, Tony, Removal of molecular assay interferences.
    상세보기

관련 콘텐츠

섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트

맨위로