초록 ▼

A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flo

A method for the multiplexed diagnostic and genetic analysis of enzymes, DNA fragments, antibodies, and other biomolecules comprises the steps of constructing an appropriately labeled beadset, exposing the beadset to a clinical sample, and analyzing the combined sample/beadset by flow cytometry. Flow cytometric measurements are used to classify, in real-time, beads within an exposed beadset and textual explanations, based on the accumulated data obtained during real-time analysis, are generated for the user. The inventive technology enables the simultaneous, and automated, detection and interpretation of multiple biomolecules or DNA sequences in real-time while also reducing the cost of performing diagnostic and genetic assays.

대표청구항 ▼

[ What is claimed is:] [1.] A method of detecting multiple analytes in a sample, said method comprising:(a) exposing a pooled population of subsets of particles to a sample, the particles in each subset having (i) one or more characteristic classification parameters that distinguish the particles of

[ What is claimed is:] [1.] A method of detecting multiple analytes in a sample, said method comprising:(a) exposing a pooled population of subsets of particles to a sample, the particles in each subset having (i) one or more characteristic classification parameters that distinguish the particles of one subset from those of another subset according to a predetermined discriminant function table, which includes fluorescence emission intensities, and (ii) a reactant specific for an analyte of interest;(b) passing the exposed pooled population of subsets of particles through an examination zone; and(c) determining the identity and quantity of each analyte of interest, if present, in the sample by substantially contemporaneously (i) collecting data relating to the one or more characteristic classification parameters, including the fluorescence emission intensities, (ii) collecting data relating to the presence or absence of a complex formed between the reactant and an analyte of interest specific to the reactant, (iii) classifying, without relying exclusively, if at all, on differences in particle size, each particle to its subset according to the predetermined discriminant function table, and (iv) quantifying the amount of complex associated with each subset.