초록 ▼

The invention relates to an apparatus and method for monitoring cells and to a method for monitoring changes in cells upon addition of an analyte to the cell's environment, comprising a device which includes an array of microelectrodes disposed in a cell culture chamber, upon which array a portion o

The invention relates to an apparatus and method for monitoring cells and to a method for monitoring changes in cells upon addition of an analyte to the cell's environment, comprising a device which includes an array of microelectrodes disposed in a cell culture chamber, upon which array a portion of cells adhere to the surfaces of the microelectrodes. The diameter of the cells are larger than the diameters of the microelectrodes. A voltage signal is applied across each of the microelectrodes and a reference electrode. Detection and monitoring of the signals resulting from the application of the voltage signal provides information regarding the electrical characteristics of the individual cells, including impedance (combined cell membrane capacitance and conductance), action potential parameters, cell membrane capacitance, cell membrane conductance, and cell/substrate seal resistance. Such an invention is useful in detecting or screening a variety of biological and chemical agents.

대표청구항 ▼

[ What is claimed is:] [1.] A method comprising the steps of:providing in a cell culture chamber a monolithic device that includes an array of planar microelectrodes disposed on a substrate, the array of microelectrodes being connected to a conductive pattern having contacts points connected thereto

[ What is claimed is:] [1.] A method comprising the steps of:providing in a cell culture chamber a monolithic device that includes an array of planar microelectrodes disposed on a substrate, the array of microelectrodes being connected to a conductive pattern having contacts points connected thereto;culturing cells in a medium provided within the cell culture chamber, wherein the diameter of a cell is larger than the diameter of a microelectrode;incubating the cells;selectively applying a signal between each microelectrode and a reference electrode disposed within the medium;detecting signals resulting from the application of the electrical signal between the microelectrodes and the reference electrode;monitoring characteristics of cells which adhere to the respective surfaces of the microelectrodes based on impedance values derived from the resulting signals, the characteristics being selected from the group consisting of impedance, action potential parameters, cell membrane capacitance, cell membrane conductance, and cell/substrate seal resistance.