초록 ▼

Apparatus for treatment of human or animal cell samples on a microscopic slide includes an element having a recessed face defined by rails which are adapted to slide on surface of the slide. An opening at the leading edge of the element has a bevelled edge and provides access to a cavity formed betw

Apparatus for treatment of human or animal cell samples on a microscopic slide includes an element having a recessed face defined by rails which are adapted to slide on surface of the slide. An opening at the leading edge of the element has a bevelled edge and provides access to a cavity formed between the face and the surface of the slide when the rails bear on the slide. The slide is tilted slightly and a vacuum nozzle is arranged at the lower edge of the slide. An end stop may be clamped to the end of the slide opposite to the end where the nozzle is located and has a bevelled edge complimentary to the bevelled edge of the element for closing the opening. A method of spreading liquid over the surface and the sample involves placing the rails on the surface with the cavity displaced laterally from the sample and the face and surface parallel, dispensing the liquid onto the surface and moving the element relative to the surface whereby the liquid becomes trapped in the cavity, and is spread evenly over the sample. The method also involves removing excess liquid after incubation by exposing the cavity to the vacuum nozzle by sliding the element over the lower edge of the slide and allowing the nozzle to suck the excess liquid away. Further treatment liquid may be added adjacent to the opening during the suction process to provide a second treatment without exposing the sample to air between treatments.

대표청구항 ▼

[ I claim:] [1.] A method of spreading liquid onto a flat surface supporting a human or animal cell sample on a portion of the surface, said method comprising the steps of:i) placing an element having a flat face adjacent the surface so that the flat face is parallel with, in close proximity to, and

[ I claim:] [1.] A method of spreading liquid onto a flat surface supporting a human or animal cell sample on a portion of the surface, said method comprising the steps of:i) placing an element having a flat face adjacent the surface so that the flat face is parallel with, in close proximity to, and displaced laterally from the sample, the flat face being spaced at a defined distance from the surface by space means;ii) dispensing the liquid onto the surface or the element; andiii) after said step of dispensing the liquid, moving the element relative to the surface, while the element is in contact with the surface via the spacer means, until the flat face of the element covers the sample and the liquid becomes trapped in a cavity defined between the surface and the flat face and is spread evenly over the sample.