초록 ▼

The present invention relates to the preparation of novel polyphenol fractions of Camellia sinensis (tea), the use thereof and formulations containing them. The invention relates specifically to the preparation of extracts deprived of caffeine but containing the polyphenols deriving from epigallocat

The present invention relates to the preparation of novel polyphenol fractions of Camellia sinensis (tea), the use thereof and formulations containing them. The invention relates specifically to the preparation of extracts deprived of caffeine but containing the polyphenols deriving from epigallocatechin in a natural ratio. The use of these novel extracts, alone or in combination with other active principles, is of interest to the food, pharmaceutical, and cosmetic industry, especially to treat cytotoxic and oxidative conditions.

대표청구항 ▼

[ We claim:] [1.] A process for extracting polyphenol fractions from Camellia sinensis, which fractions cumulatively contain no more than about 0.2% by weight of caffeine, said process comprising:(a) providing a vegetable biomass of Camellia sinensis containing a quantity of polyphenols;(b) extracti

[ We claim:] [1.] A process for extracting polyphenol fractions from Camellia sinensis, which fractions cumulatively contain no more than about 0.2% by weight of caffeine, said process comprising:(a) providing a vegetable biomass of Camellia sinensis containing a quantity of polyphenols;(b) extracting the vegetable biomass with a hydrated organic solvent;(c) at least partially evaporating the solvent;(d) adding to a residue produced upon said at least partial evaporation an aqueous mixture of from about 30-60% by volume of methanol to form a hydromethanol suspension of said residue;(e) extracting the hydromethanol suspension with at least one chlorinated solvent;(f) filtering and then concentrating the hydromethanol phase obtained upon extraction with said at least one chlorinated solvent;(g) extracting the concentrated hydromethanol phase with at least one solvent selected from the group consisting of aliphatic esters, alcohols and ketones wherein said at least one solvent is substantially immiscible with the hydromethanol phase;(h) concentrating the solvent phase from the immediately preceding extraction step;(i) diluting said concentrated solvent phase with at least one chlorinated solvent;(j) treating said diluted solvent phase with at least one sulfonic acid resin in an anhydrous medium to remove additional caffeine; and(k) isolating said polyphenol fractions from said diluted solvent phase to permit recovery of said fractions substantially free of said solvent, wherein each said extraction step is carried out at substantially ambient temperature.