초록 ▼

This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In

This invention provides nucleic acid affinity matrices that bear a large number of different nucleic acid affinity ligands allowing the simultaneous selection and removal of a large number of preselected nucleic acids from the sample. Methods of producing such affinity matrices are also provided. In general the methods involve the steps of a) providing a nucleic acid amplification template array comprising a surface to which are attached at least 50 oligonucleotides having different nucleic acid sequences, and wherein each different oligonucleotide is localized in a predetermined region of said surface, the density of said oligonucleotides is greater than about 60 different oligonucleotides per 1 cm.sup.2, and all of said different oligonucleotides have an identical terminal 3' nucleic acid sequence and an identical terminal 5' nucleic acid sequence. b) amplifying said multiplicity of oligonucleotides to provide a pool of amplified nucleic acids; and c) attaching the pool of nucleic acids to a solid support.

대표청구항 ▼

[ What is claimed is:] [1.] A method of making a nucleic acid affinity matrix for removing known nucleic acid molecules in a sample, said method comprising the steps of:i) providing a multiplicity of nucleic acid probes selected such that for each of said known nucleic acid molecules there exists in

[ What is claimed is:] [1.] A method of making a nucleic acid affinity matrix for removing known nucleic acid molecules in a sample, said method comprising the steps of:i) providing a multiplicity of nucleic acid probes selected such that for each of said known nucleic acid molecules there exists in said multiplicity a probe sufficiently complementary to said nucleic molecule or a subsequence thereof to hybridize thereto under stringent conditions, and wherein said multiplicity of probes does not include every possible probe having the same length as said probe;ii) attaching said probes to a solid support;iii) contacting the multiplicity of probes with the sample, whereby the known nucleic acid molecules hybridize to the multiplicity of probes; andiv) analyzing a nucleic acid molecule remaining in the sample.