초록 ▼

Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). Th

Improved liquid chromatography systems having components made of titanium, stainless steel, or organic polymeric material are useful in the separation of polynucleotide fragments, particularly large fragments of double-stranded polynucleotides, by Matched Ion Polynucleotide Chromatography (MIPC). The titanium, stainless steel, or polymeric components are treated so that they do not release multivalent cations into aqueous solutions flowing through the chromatography system. Alternatively, or in addition to utilizing materials made of the components listed above, a multivalent cation capture resin placed upstream of the separation column can be employed to remove multivalent ions from the system. The multivalent cation capture resin can be contained in a guard disk, a guard column, or a guard cartridge. Novel methods for separating mixtures of polynucleotide fragments into fractions based on their molecular weight by Matched Ion Polynucleotide Chromatography and slalom chromatography utilize the liquid chromatographic systems described above.

대표청구항 ▼

[ What is claimed is:] [1.] A system for separating a mixture of polynucleotide fragments comprising a chromatographic column having two ends, said column containing a separation bed of Matched Ion Polynucleotide Chromatography separation particles held in the column between porous frits positioned

[ What is claimed is:] [1.] A system for separating a mixture of polynucleotide fragments comprising a chromatographic column having two ends, said column containing a separation bed of Matched Ion Polynucleotide Chromatography separation particles held in the column between porous frits positioned at each end thereof, said column having an inlet, an injection valve in communication with said inlet through a flow path therebetween, mobile phase supply means in communication with said injection valve through at least one flow path therebetween, and multivalent cation capture resin, selected from cation exchange resin and chelating resin, positioned in said flow path between said inlet and said injection valve, said multivalent cation capture resin being capable of removing multivalent cations from aqueous solutions, whereby any multivalent cation contaminants in said flow path are removed before said contaminants contact the separation bed wherein said injection valve communicates with the inlet of said separation column through a conduit, and said mobile phase supply means communicates with said injection through at least one conduit, and wherein said porous frits, chromatographic column, injection valve, mobile phase supply means, and conduits have process solution-contacting surfaces which contact process solutions held therein or flowing therethrough, and wherein the process solution-contacting surfaces of said chromatographic column, injection valve, mobile phase supply means, and conduits are material which does not release multivalent cations into aqueous solutions held therein or flowing therethrough.