초록 ▼

A universal process is disclosed for extracting and purifying nucleic acids from extremely small amounts of highly contaminated various biological and other starting materials. The invention has applications in forensic medicine, medical diagnosis, molecular biology, biochemistry, genetic technology

A universal process is disclosed for extracting and purifying nucleic acids from extremely small amounts of highly contaminated various biological and other starting materials. The invention has applications in forensic medicine, medical diagnosis, molecular biology, biochemistry, genetic technology and all related fields. The process is characterized in that nucleic acid-containing materials are lysed, the lysate is incubated with a non-porous, non-structured, highly disperse, homogeneous and chemically pure SiO.sub.2 substrate, the substrate is isolated with the bound nucleic acids and washed with a buffer solution, then the nucleic acids are dissolved from the substrate by a buffer with a lower salt concentration. Lysis of the material and nucleic acid immobilization are preferably carried out in a reaction vessel. The substrate particles have a size of 7-40 nm, preferably 40 nm, and a specific surface from 50-300 g/m.sup.2, preferably 50 g/m.sup.2.

대표청구항 ▼

[ We claim:] [1.] A universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated biological starting materials bya) lysis of the biological starting materials containing nucleic acids, with buffers containing at least one chaotropic salt of high ion

[ We claim:] [1.] A universal process for isolating and purifying nucleic acids from extremely small amounts of highly contaminated biological starting materials bya) lysis of the biological starting materials containing nucleic acids, with buffers containing at least one chaotropic salt of high ionic strength,b) incubation with a highly disperse, non-porous, unstructured and homogeneous silica carrier, the silica particles having a particle size of 7 to 40 nm and an active surface area of 50 to 300 m.sup.2 /g,c) separation of the nucleic acids fixed to the carrier, from the lysate,d) washing the nucleic acids fixed to the surface of the carrier, with a washing buffer ande) eluting the nucleic acids from the carrier with a buffer of low salt concentration,wherein the biological starting materials are chosen from the group consisting of less and 0.5 mg of tissue material, less than 0.5 .mu.L blood or blood traces on clothing, less than 5 .mu.L of saliva, and fewer than 10.sup.3 cells of different biological starting materials.