|국가/구분||United States(US) Patent 등록|
|국제특허분류(IPC7판)||C12N-011/10 C12N-011/02 B09B-003/00|
|미국특허분류(USC)||435/178 ; 435/177 ; 435/262.5|
|발명자 / 주소|
|출원인 / 주소|
|대리인 / 주소||
|인용정보||피인용 횟수 : 41 인용 특허 : 3|
Microorganisms which may be in the form of homogenated anaerobic sludge are immobilized in a polymeric gel matrix containing chitosan and lignosulphonate. A mixture of chitosan solution and a microorganism is added dropwise to a solution of lignosulphonate to crosslink chitosan droplets and form beads of chitosan/lignosulphonate matrix membrane encapsulating the microorganism. Alternatively, the mixture is added to a water-insoluble organic phase to form chitosan beads in an emulsion, and to the emulsion is added a lignosulphonate solution to crosslink t...
[ We claim:] [1.] A method for the immobilization of a microorganism, comprising(a) providing an aqueous solution of chitosan and an acid, wherein the concentration of chitosan is 1-1.5%/ w/v,(b) adding a microorganism, to the solution, while maintaining the pH in a range of 4.0 to 5.0 to form a mixture,(c) adding the mixture dropwise to an aqueous solution of lignosulphonate, wherein the concentration of lignosulphonate is 8-12% w/v, while maintaining the pH in the range of 7.0 to 8.0 by means of a buffer, to form beads of a chitosan/lignosulphonate mat...