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특허 상세정보

Microorganisms immobilized in chitosan crosslinked with lignosulphonate for purification of waste water

국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) C12N-011/10    C12N-011/02    B09B-003/00   
미국특허분류(USC) 435/178 ; 435/177 ; 435/262.5
출원번호 US-0934571 (1997-09-19)
발명자 / 주소
출원인 / 주소
대리인 / 주소
    Anderson
인용정보 피인용 횟수 : 41  인용 특허 : 3
초록

Microorganisms which may be in the form of homogenated anaerobic sludge are immobilized in a polymeric gel matrix containing chitosan and lignosulphonate. A mixture of chitosan solution and a microorganism is added dropwise to a solution of lignosulphonate to crosslink chitosan droplets and form beads of chitosan/lignosulphonate matrix membrane encapsulating the microorganism. Alternatively, the mixture is added to a water-insoluble organic phase to form chitosan beads in an emulsion, and to the emulsion is added a lignosulphonate solution to crosslink t...

대표
청구항

[ We claim:] [1.] A method for the immobilization of a microorganism, comprising(a) providing an aqueous solution of chitosan and an acid, wherein the concentration of chitosan is 1-1.5%/ w/v,(b) adding a microorganism, to the solution, while maintaining the pH in a range of 4.0 to 5.0 to form a mixture,(c) adding the mixture dropwise to an aqueous solution of lignosulphonate, wherein the concentration of lignosulphonate is 8-12% w/v, while maintaining the pH in the range of 7.0 to 8.0 by means of a buffer, to form beads of a chitosan/lignosulphonate mat...

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