초록 ▼

Method of detecting a nucleic acid by converting the nucleic acid into a single-stranded nucleic acid under alkaline conditions, wherein at least one detergent from the group of anionic, non-ionic and zwitterionic detergents is present, adding an immobilizable or immobilized capture probe, hybridizi

Method of detecting a nucleic acid by converting the nucleic acid into a single-stranded nucleic acid under alkaline conditions, wherein at least one detergent from the group of anionic, non-ionic and zwitterionic detergents is present, adding an immobilizable or immobilized capture probe, hybridizing the capture probe with the nucleic acid under immobilization of the nucleic acid via the capture probe and detecting the amount of synthesized, immobilized hybrid.

대표청구항 ▼

[ We claim:] [1.] A method for the detection of nucleic acids in different samples, comprising the steps ofa) amplifying nucleic acids in any samples to be tested,b) adding at least one detergent selected from the group consisting of anionic, non-ionic and zwitterionic detergents to said amplified n

[ We claim:] [1.] A method for the detection of nucleic acids in different samples, comprising the steps ofa) amplifying nucleic acids in any samples to be tested,b) adding at least one detergent selected from the group consisting of anionic, non-ionic and zwitterionic detergents to said amplified nucleic acids under alkaline conditions to form reaction mixtures, said detergents being present in a stabilizing amount between 0.01 wt % and 10 wt %, wherein said alkaline conditions are at a pH between 10 and 14,c) incubating said reaction mixtures in plastic vessels at least until any double stranded nucleic acids present in the reaction mixtures are converted into single stranded nucleic acids,d) adding immobilizable or immobilized capture probes to said reaction mixtures,e) hybridizing said single stranded nucleic acids with said capture probes to form immobilized hybrids, andf) determining the amount of immobilized hybrids.