초록 ▼

The present invention discloses devices and methods of performing high throughput screening of the physiological response of cells to biologically active compounds and methods of combining high-throughput with high-content spatial information at the cellular and subcellular level as well as temporal

The present invention discloses devices and methods of performing high throughput screening of the physiological response of cells to biologically active compounds and methods of combining high-throughput with high-content spatial information at the cellular and subcellular level as well as temporal information about changes in physiological, biochemical and molecular activities. The present invention allows multiple types of cell interactions to be studied simultaneously by combining multicolor luminescence reading, microfluidic delivery, and environmental control of living cells in non-uniform micro-patterned arrays.

대표청구항 ▼

[ What is claimed is:] [1.] A method for producing a cassette for cell screening, comprising:a) providing a base with a surfaceb) preparing a micro-patterned chemical array;c) modifying the micro-patterned chemical array to produce a modified micro-patterned chemical array comprising multiple differ

[ What is claimed is:] [1.] A method for producing a cassette for cell screening, comprising:a) providing a base with a surfaceb) preparing a micro-patterned chemical array;c) modifying the micro-patterned chemical array to produce a modified micro-patterned chemical array comprising multiple different cell binding sites on the surface of the base, wherein the different cell binding sites interact with different cell types, wherein a cell binding site comprises a well;d) binding cells to the modified micro-patterned chemical array to produce an ordered array of cell types seeded on the wells; ande) providing a fluid delivery system for delivering a combinatorial of reagents to the ordered array of cell types; wherein said fluid delivery system comprises a chamber that mates with the base containing the ordered array of cell types, wherein the chamber comprises:(i) etched domains matching the wells on the surface of the base, and(ii) microfluidic channels that supply fluid to the etched domains.