초록 ▼

A method and apparatus for forming microarrays of biological samples on a support are disclosed. The method involves dispensing a known volume of a reagent at each selected array position, by tapping a capillary dispenser on the support under conditions effective to draw a defined volume of liquid o

A method and apparatus for forming microarrays of biological samples on a support are disclosed. The method involves dispensing a known volume of a reagent at each selected array position, by tapping a capillary dispenser on the support under conditions effective to draw a defined volume of liquid onto the support. The apparatus is designed to produce a microarray of such regions in an automated fashion.

대표청구항 ▼

[ It is claimed:] [1.] A method of forming a microarray of discrete analyte-assay regions on a solid support, where each discrete region in the microarray has a select(d, analyte-specific reagent, said method comprising,(a) loading a solution of a selected analyte-specific reagent in a reagent-dispe

[ It is claimed:] [1.] A method of forming a microarray of discrete analyte-assay regions on a solid support, where each discrete region in the microarray has a select(d, analyte-specific reagent, said method comprising,(a) loading a solution of a selected analyte-specific reagent in a reagent-dispensing device having an elongate capillary channel adapted to hold a quantity of the reagent solution and having a tip region at which the channel is open-sided and the solution in the channel forms a meniscus,(b) tapping the tip of the dispensing device against a solid support at a defined position on the surface, with an impulse effective to break the meniscus in the capillary channel and deposit a selected volume of solution on the surface, and(c) repeating steps (a) and (b) until said microarray is formed.