초록 ▼

Methods for the determination of nuclease stability of an oligomeric compound and its deletion sequences by capillary gel electrophoresis using laser-induced fluorescence detection (LIF CGE) are provided. Fluorescently labeled oligomeric compounds are treated with one or more agents having nuclease

Methods for the determination of nuclease stability of an oligomeric compound and its deletion sequences by capillary gel electrophoresis using laser-induced fluorescence detection (LIF CGE) are provided. Fluorescently labeled oligomeric compounds are treated with one or more agents having nuclease activity resulting in an assay mixture of the original oligomeric compound and its deletion sequences. A diluted aliquot taken directly from the assay mixture is analyzed using LIF CGE. Results of the assay yield quantitative concentrations of the oligomeric compound and each of the deletion sequences. In further embodiments, the invention provides methods for determining the relative binding affinity of one or more oligomeric compounds for a substrate having nuclease activity, and methods for determining the nuclease activity of an enzyme.

대표청구항 ▼

[ What is claimed is:] [1.] A method for determining the nuclease stability of an oligomeric compound comprising:(a) digesting said oligonucleotide with a nuclease;(b) performing capillary electrophoresis on the product of said digestion; and(c) detecting and quantifying at least some components of

[ What is claimed is:] [1.] A method for determining the nuclease stability of an oligomeric compound comprising:(a) digesting said oligonucleotide with a nuclease;(b) performing capillary electrophoresis on the product of said digestion; and(c) detecting and quantifying at least some components of said digestion;thereby determining said nuclease stability of said oligomeric compound;wherein said components are detected using laser-induced fluorescence.