초록 ▼

The invention relates to a method of analysing a chemical or physical interaction taking place in a film layer at an optical sensor surface when the film layer is contacted with a fluid sample containing a species capable of interacting with the film layer, wherein the interaction is monitored by de

The invention relates to a method of analysing a chemical or physical interaction taking place in a film layer at an optical sensor surface when the film layer is contacted with a fluid sample containing a species capable of interacting with the film layer, wherein the interaction is monitored by determination of the refractive index of the film layer through a light-intensity signal producing technique by measuring the relationship between a parameter of the incident and/or reflected light and one of the minimum, the maximum and the centroid of the light intensity signal curve. The method is characterized by also monitoring the variation of the light intensity signal value at said minimum, maximum or centroid with time to determine at least one of (i) the degree of homogeneity of sample species concentration in the sensed film layer volume to determine therefrom when the interaction is mass transport limited and when the interaction is kinetically controlled, a varying light intensity signal level indicating mass transport limitation and a stabilized light intensity signal level indicating kinetic control; and (ii) the degree of resulting homogeneity/heterogeneity in the sensed film layer volume during or after the interaction, a changed stabilized light intensity signal level indicating and corresponding to the degree of a change in homogeneity/heterogeneity.

대표청구항 ▼

[ We claim:] [1.] A method of analyzing a chemical or physical interaction taking place in a film layer at an optical sensor surface when the film layer is contacted with a fluid sample containing a species capable of interacting with the film layer, wherein the interaction is monitored by determini

[ We claim:] [1.] A method of analyzing a chemical or physical interaction taking place in a film layer at an optical sensor surface when the film layer is contacted with a fluid sample containing a species capable of interacting with the film layer, wherein the interaction is monitored by determining the refractive index of the film layer through a light-intensity signal producing technique that involves measuring the relationship between a parameter associated with the incident or reflected light and one of the minimum, the maximum and the centroid of the light intensity signal curve, wherein the method comprises the step of monitoring the variation of the light intensity signal value at the minimum, maximum or centroid with time to determine at least one of (i) the degree of homogeneity of sample species concentration in the sensed film layer volume to determine therefrom when the interaction is mass transport limited and when the interaction is kinetically controlled; and (ii) the degree of resulting homogeneity/heterogeneity in the sensed film layer volume during or after the interaction.