초록 ▼

Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the ste

Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.

대표청구항 ▼

[ What is claimed is:] [1.] An apparatus for detecting fluorescently marked regions on a surface of a substrate, said apparatus comprising:a light source;an optical train for directing light from said light source at said substrate;a focusing system for focusing said light onto a first surface of sa

[ What is claimed is:] [1.] An apparatus for detecting fluorescently marked regions on a surface of a substrate, said apparatus comprising:a light source;an optical train for directing light from said light source at said substrate;a focusing system for focusing said light onto a first surface of said substrate;a detector for detecting fluoresced light from said fluorescently marked regions in response to said light; anda flow cell comprising a mounting surface with a cavity therein having said substrate thereon, wherein there is a sealed relationship between said mounting surface and said substrate, whereby said surface of said substrate is in fluid communication with said cavity, said cavity having an inlet and an outlet, and said inlet being connected to a pump for transferring materials into said cavity through said inlet and out of said cavity through said outlet.