초록 ▼

The present invention provides methods for the efficient production of transformants with low transgene copy numbers. In the method, the average transgene copy number of transformants is decreased through methods which are believed to limit the interaction between segments of transforming DNA prior

The present invention provides methods for the efficient production of transformants with low transgene copy numbers. In the method, the average transgene copy number of transformants is decreased through methods which are believed to limit the interaction between segments of transforming DNA prior to transformation. The methods comprise means for end-modification of transforming DNA and use of limited quantities of DNA for transforrnation. Production of single or low copy transformation events is desirable in that it avoids many of the problems associated with high transgene copy number including co-suppression, unpredictable gene expression and non-Mendelian inheritance.

대표청구항 ▼

[ What is claimed is:] [1.] A method of preparing a transgenic plant comprising:(a) obtaining a linear nucleic acid segment;(b) end-modifying said linear nucleic acid segment;(c) contacting a recipient plant cell with said linear nucleic acid segment; and(d) regenerating a plant from a plant cell wh

[ What is claimed is:] [1.] A method of preparing a transgenic plant comprising:(a) obtaining a linear nucleic acid segment;(b) end-modifying said linear nucleic acid segment;(c) contacting a recipient plant cell with said linear nucleic acid segment; and(d) regenerating a plant from a plant cell which has been stably transformed with said linear nucleic acid segment. [46.] A method of transforming a maize plant comprising:(a) preparing a microprojectile composition comprising from 1 ng to 2000 ng of DNA per each 1.8 mg of starting microprojectiles;(b) contacting recipient maize cells with said microprojectile composition;(c) regenerating plants from recipient maize cells which have received said DNA;(d) identifying a fertile transgenic maize plant the genomc of which has been augmented relative to that of the corresponding nontransgenic recipient maize plant through the stable introduction of said DNA.