초록 ▼

The present invention relates to devices and methods for the collection, storage, and purification of nucleic acids, such as DNA or RNA, from fluid samples for subsequent genetic characterization, primarily by conventional amplification methods. The present invention can be used to collect, store, o

The present invention relates to devices and methods for the collection, storage, and purification of nucleic acids, such as DNA or RNA, from fluid samples for subsequent genetic characterization, primarily by conventional amplification methods. The present invention can be used to collect, store, or purify nucleic acids either from a biological source other than untreated whole blood, the biological source having naturally occurring nucleic acid amplification inhibitors present, (including either a buccal swab, cerebrospinal fluid, feces, lymphatic fluid, a plasma sample, a saliva sample, a serum sample, urine, or a suspension of cells or viruses), or from a treated whole blood source that has naturally occurring nucleic acid amplification inhibitors present, as well as added blood stabilization components that also inhibit nucleic acid amplification. More importantly, these nucleic acids can be released after collection or storage in a manner that enables them to be amplified by conventional techniques such as polymerase chain reaction. In particular, an absorbent material that does not bind nucleic acids irreversibly is impregnated with a chaotropic salt. A biological source sample is contacted with the impregnated absorbent material. Any nucleic acids present in the biological source can be either eluted or resolubilized off the absorbent material.

대표청구항 ▼

[ We claim:] [1.] A device for storing and purifying nucleic acids from a biological source other than untreated whole blood, the biological source having naturally occurring nucleic acid amplification inhibitors present, comprising an absorbent material that does not bind irreversibly to nucleic ac

[ We claim:] [1.] A device for storing and purifying nucleic acids from a biological source other than untreated whole blood, the biological source having naturally occurring nucleic acid amplification inhibitors present, comprising an absorbent material that does not bind irreversibly to nucleic acids, a chaotropic salt impregnated about the absorbent material, nucleic acids from the biological source releasably bound to the absorbent in an amplifiable form, the amplification inhibitors being bound to the adsorbent such that resolubilization does not occur by releasing the nucleic acids from the adsorbent.