초록 ▼

The invention disclosed is particularly directed to a process for the purification of DNA, and is especially useful for the isolation of biologically active plasmid DNA. The process is initiated by binding the DNA to silicon carbide particles, either in the presence or absence of chaotropic agents.

The invention disclosed is particularly directed to a process for the purification of DNA, and is especially useful for the isolation of biologically active plasmid DNA. The process is initiated by binding the DNA to silicon carbide particles, either in the presence or absence of chaotropic agents. The bound DNA is subsequently eluted from the resin in low salt buffer or water. The resulting purified DNA is substantially free of proteins and is shown to be biologically active. The scope and diversity of the process is demonstrated here since this process can be used for the isolation of RNA and genomic DNA as well as the purification of PCR products and isolation of DNA from agarose gel slices.

대표청구항 ▼

[ We claim:] [1.] A method for the isolation of a nucleotide polymer from a sample comprising the steps of:adding silicon carbide to the sample and mixing for binding of the nucleotide polymer to the silicon carbide;separating the silicon carbide with the bound nucleotide polymer from the mixture ob

[ We claim:] [1.] A method for the isolation of a nucleotide polymer from a sample comprising the steps of:adding silicon carbide to the sample and mixing for binding of the nucleotide polymer to the silicon carbide;separating the silicon carbide with the bound nucleotide polymer from the mixture obtained;eluting the nucleotide polymer from the silicon carbide.