초록 ▼

The invention relates to a microfabricated device and methods of using the device for analyzing and sorting of single polynucleotides, e.g. by size, according to an optical signal measured within a detection region of the device. An optical signal such as fluorescence from a reporter molecule associ

The invention relates to a microfabricated device and methods of using the device for analyzing and sorting of single polynucleotides, e.g. by size, according to an optical signal measured within a detection region of the device. An optical signal such as fluorescence from a reporter molecule associated with the polynucleotide molecules can be used to determine polynucleotide size or to direct selected polynucleotides into one or more selected branch channels of the device.

대표청구항 ▼

[ It is claimed:] [1.]1. A device for processing a flow of polynucleotide molecules comprising a chip having a substrate and an analysis unit, wherein the analysis unit comprises:a) a main channel having a polynucleotide sample inlet, a detection region downstream of the sample inlet, and a branch p

[ It is claimed:] [1.]1. A device for processing a flow of polynucleotide molecules comprising a chip having a substrate and an analysis unit, wherein the analysis unit comprises:a) a main channel having a polynucleotide sample inlet, a detection region downstream of the sample inlet, and a branch point discrimination region adjacent to and downstream of the detection region, wherein on average one polynucleotide molecule at a time is placed within the detection region;(b) at least two branch channels originating at the branch point discrimination region and in communication with the main channel;(c) a detector sensitive to each polynucleotide molecule passing through the detection region; and(d) a flow control responsive to the detector and acting to direct each polynucleotide molecules at the discrimination region into a selected branch channel.