초록 ▼

The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the

The invention describes a method for the isolation of components from samples, particularly large molecular weight DNA from biological samples. The method involves the application of controlled oscillatory mechanical energy to the sample for short periods of time of about 5 to 60 seconds to lyse the sample and release the component(s) from the sample, followed by standard isolation methods. In preferred embodiments, the method includes the use of a spherical particle for applying the mechanical energy.

대표청구항 ▼

[ What is claimed is:] [1.]1. A method of isolating high molecular weight nucleic acid from a biological material which comprises mechanically releasing said high molecular weight nucleic acid from said material by the application of rapidly oscillating reciprocal mechanical energy to said material

[ What is claimed is:] [1.]1. A method of isolating high molecular weight nucleic acid from a biological material which comprises mechanically releasing said high molecular weight nucleic acid from said material by the application of rapidly oscillating reciprocal mechanical energy to said material in the presence of a liquid medium in a closed container to produce a released high molecular weight nucleic acid solution, wherein said released high molecular weight nucleic acid has an average molecular weight greater than 10 kilobases, said liquid medium contains one or more particles and detergent in a amount of from about 0.1% to 10% weight per weight (w/w), and said application of said energy is conducted by subjecting said container and thereby said material to oscillations at an oscillatory rate of between about 25 hertz (Hz) to about 166 Hz for a period of time of between about 3 seconds to about 5 minutes.