초록 ▼

The separation device of the invention is directed to a container having an interior surface defining a channel. The container further has an inlet and an outlet. On the interior surface of the channel are pole tips which may be in a sawtooth configuration having sharp angles facing the interior of

The separation device of the invention is directed to a container having an interior surface defining a channel. The container further has an inlet and an outlet. On the interior surface of the channel are pole tips which may be in a sawtooth configuration having sharp angles facing the interior of the channel that generate a high gradient magnetic field in the channel. Within the channel may be incorporated separating material. The separating material eliminates the direct contact of cells with the magnetic pole material. The separating material, as well as the sawtooth pole tips also serves the purpose of creating a field gradient across the entire container to avoid the problem of zero field gradient in the center of the container where the velocity is greatest, and where more cells flow. The separating material is designed to cause a substantially unobstructed flow of medium through the channel so that unlabeled substances are not trapped in the separating material.

대표청구항 ▼

[ What is claimed is:] [18.]18. A method for separating magnetically labeled substances from a medium comprising:introducing a medium of magnetically labeled substances and unlabeled substances to a container having an interior channel that permits substantially unobstructed flow of medium therethro

[ What is claimed is:] [18.]18. A method for separating magnetically labeled substances from a medium comprising:introducing a medium of magnetically labeled substances and unlabeled substances to a container having an interior channel that permits substantially unobstructed flow of medium therethrough;exposing the medium of magnetically labeled substances and unlabeled substances to a high gradient magnetic field in the channel, the channel having sharply angled pole tips, in a saw-tooth configuration, arranged asymmetrically on substantially opposite sides of the channel, the saw-tooth pole tips creating the high gradient magnetic field; wherein the saw-tooth pole tips create a continuous magnetic field across the channel such that a zero magnetic field gradient does not exist in the channel;retaining magnetically labeled substances in the high gradient magnetic field; andremoving substantially all unlabeled substances from the channel with the medium.