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특허 상세정보

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Human embryonic germ cell line and methods of use

특허상세정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) C12N-005/02    C12N-005/00    C12N-005/08   
미국특허분류(USC) 435/384 ; 435/383 ; 435/366
출원번호 US-0052772 (1998-03-31)
발명자 / 주소
  • Gearhart John D.
  • Shamblott Michael Joseph
출원인 / 주소
  • The Johns Hopkins University School of Medicine
대리인 / 주소
    Fish & Richardson P.C.
인용정보 피인용 횟수 : 62  인용 특허 : 20
초록

Primordial germ cells isolated from human embryonic tissue, such as from the gonadal ridges of human embryo, are disclosed. The primordial germ cells are cultured resulting in cells that resemble embryonic stem cells or embryonic germ cells in morphology and pluripotency. The cells are maintained several months in culture and can be genetically manipulated using transgenic technology to insert heterologous genetic material.

대표
청구항
▼

[ What is claimed is:] [1.]1. A method for producing human pluripotent embryonic germ (hEG) cells, comprising culturing human primordial germ cells (PGCs) in a culture medium comprising:(a) a ligand which binds to a receptor which can associate with glycoprotein 130 (gp 130); and(b) a growth factor;until cells with the morphology of human pluripotent embryonic germ cells are observed, and wherein the cells exhibit the following culture characteristics during maintenance: dependence on a ligand that binds to a receptor, which can associate with gp 130; an...

이 특허에 인용된 특허 (20)

  1. Rosenkrans ; Jr. Charles F. (111 Ridge Dr. Mt. Horeb WI 53572) Keefer Carol L. (2204 Beatrice Ct. Madison WI) Sims Michelle M. (2204 Beatrice Ct. Stoughton WI 53589), Bovine embryo medium.
  2. Caplan Arnold I. ; Haynesworth Stephen E., Connective tissue regeneration using human mesenchymal stem cell preparations.
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  6. Ronnett Gabriele V. (Baltimore MD) Nye Jeffrey S. (New York NY) Hester Lynda D. (Towson MD) Snyder Solomon H. (Baltimore MD), Human neuronal cell line.
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  8. Anderson David J. (Altadena CA) Stemple Derek L. (Newton MA), Immoralized neural crest stem cells and methods of making.
  9. Anderson David J. ; Stemple Derek L., In vitro method for obtaining an isolated population of mammalian neural crest stem cells.
  10. Williams Robert L. (Warrndyte AUX) Gough Nicholas M. (North Balwyn AUX) Hilton Douglas J. (Warrandyte AUX), In vitro propagation of embryonic stem cells.
  11. Anderson David J. (Altadena CA) Stemple Derek L. (Pasadena CA), Mammalian neural crest stem cells.
  12. Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI), Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultur.
  13. Emerson Stephen G. (Ann Arbor MI) Clarke Michael F. (Ann Arbor MI) Palsson Bernhard O. (Ann Arbor MI) Schwartz Richard M. (Ann Arbor MI), Methods and compositions for the ex vivo replication of human hematopoietic stem cells.
  14. Hill Beth Louis ; Rozler Elen ; Chen Benjamin P., Methods of obtaining compositions enriched for hematopoietic stem cells, antibodies for use therein, compositions derive.
  15. Hogan Brigid L. M. (Brentwood TN), Pluripotential embryonic cells and methods of making same.
  16. Hogan Brigid L. M. (Brentwood TN), Pluripotential embryonic stem cells and methods of making same.
  17. Hogan Brigid L. M. (Brentwood TN), Pluripotential embryonic stem cells and methods of making same.
  18. Thomson James A., Primate embryonic stem cells.
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  20. Wheeler Matthew B. (Tolono IL), Transgenic swine compositions and methods.

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  7. Janjic, Jelena; Ahrens, Eric T., Compositions and methods for producing cellular labels for nuclear magnetic resonance techniques.
  8. Xu, Chunhui, Compound screening using cardiomyocytes.
  9. Xu, Chunhui; Gold, Joseph D., Conditioned media for propagating human pluripotent stem cells.
  10. Thomson,James A.; Levenstein,Mark, Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium.
  11. Amit, Michal; Itskovitz-Eldor, Joseph, Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state.
  12. Leonhardt, Howard J; Lashinski, Robert D., Deployment system for myocardial cellular material.
  13. Gearhart, John D.; Shamblott, Michael Joseph, Differentiation of human embryonic germ cells.
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  15. Xu, Chunhui, Differentiation protocol for making human cardiomyocytes.
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  21. Lin, Shi-Lung; Ying, Shao-Yao; Wu, David Ts, Generation of human embryonic stem-like cells using intronic RNA.
  22. Mandalam, Ramkumar; Xu, Chunhui; Gold, Joseph D.; Carpenter, Melissa K., Human embryonic stem cells genetically modified to contain a nucleic acid and cultured with fibroblast growth factor.
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  24. Allen-Hoffmann, B. Lynn; Centanni, John M., Kit for species specific DNA detection.
  25. Westlund, Randy; Ross, Jeffrey, Lead electrode incorporating extracellular matrix.
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  33. Ross, Jeffrey; Spinelli, Julio C., Method and apparatus for modulating cellular growth and regeneration using ventricular assist device.
  34. Girouard, Steven D.; Ross, Jeffrey; Salo, Rodney W., Method and apparatus to modulate cellular regeneration post myocardial infarct.
  35. Girouard, Steven D.; Ross, Jeffrey, Method for preparing an implantable controlled gene or protein delivery device.
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  38. Kataoka, Masaki; Fukunaga, Hideki; Inoue, Hiroshi; Nishimura, Yuji; Hirakata, Susumu; Imazeki, Atsumichi, Method of producing liquid droplet ejection head.
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  50. Gliner, Bradford Evan, Neural stimulation system and method responsive to collateral neural activity.
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  53. Xu, Chunhui; Gold, Joseph D., Process for making transplantable cardiomyocytes from human embryonic stem cells.
  54. Smith, Austin Gerard; Burdon, Thomas Grant, Propagation and/or derivation of embryonic stem cells.
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  57. Allen Hoffmann,Lynn; Centanni,John M., Species specific DNA detection.
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