초록 ▼

A method of analyzing cells in a carrier solution comprises the following steps: (a) Introducing the carrier solution into a conduit having a surface portion (preferably a substantially flat surface portion). The carrier solution has the cells suspended therein. (b) Allowing the cells to settle on t

A method of analyzing cells in a carrier solution comprises the following steps: (a) Introducing the carrier solution into a conduit having a surface portion (preferably a substantially flat surface portion). The carrier solution has the cells suspended therein. (b) Allowing the cells to settle on the surface portion, the surface portion including at least one imaging field. In an alternate embodiment, one or more discreet capture zones (e.g., formed from an affinity species immobilized on the substrate or a textured region on the substrate) are formed on the surface portion, and this step (b) comprises capturing the cells in the capture zone. (c) Sequentially interrogating a plurality of the cells in the imaging field with emitted light. (d) Processing resultant light from the imaging field. (e) Generating digital information for each of the plurality of cells from the resultant light. (f) Generating a response file for each of the plurality of cells from the digital information. The response file generated in the method can be used for the sizing, enumeration, characterization, and/or classification of cells in the sample. Apparatus for carrying out the foregoing method is also disclosed, along with methods and apparatus employing reciprocal flow techniques.

대표청구항 ▼

[ What is claimed is:] [1.]1. A method of analyzing cells in a carrier solution, comprising:(a) introducing said carrier solution into a conduit having a surface portion wherein the surface portion has a discreet capture zone formed therein, said carrier solution having a plurality of cells suspende

[ What is claimed is:] [1.]1. A method of analyzing cells in a carrier solution, comprising:(a) introducing said carrier solution into a conduit having a surface portion wherein the surface portion has a discreet capture zone formed therein, said carrier solution having a plurality of cells suspended therein;(b) contacting said plurality of said cells to said surface portion, said surface portion containing at least one imaging field;(c) sequentially interrogating said plurality of cells in said imaging field with at least two different types of emitted light;(d) processing resultant light from said imaging field for each of said at least two different types of emitted light;(e) generating digital information for each of said plurality of cells from said resultant light for each of said at least two different types of emitted light; and then(f) generating a response file for each of said plurality of cells from said digital information for each of said at least two different types of emitted light;and wherein said sequentially interrogating step is followed by the steps of: altering the temperature of said cells in said capture zone; and then repeating said sequentially interrogating step.