초록 ▼

The invention provides an apparatus and method for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber. The invention may be used in conducting hybridization reactions, as of biopolymers such as DNA, RNA, oligonucleotides, peptides, polypeptides, proteins, anti

The invention provides an apparatus and method for conducting chemical or biochemical reactions on a solid surface within an enclosed chamber. The invention may be used in conducting hybridization reactions, as of biopolymers such as DNA, RNA, oligonucleotides, peptides, polypeptides, proteins, antibodies, and the like. In another aspect, the invention provides an improved method for mixing a thin film of solution, as in a hybridization chamber. The invention further provides a kit for carrying out the methods of the invention.

대표청구항 ▼

[ What is claimed is:] [1.]1. A device for conducting hybridization assays within an enclosed hybridization chamber, comprising:a substrate having a surface with at least a portion of said surface representing a hybridization region, having an area of about 4 mm.sup.2 to about 500 mm.sup.2 wherein t

[ What is claimed is:] [1.]1. A device for conducting hybridization assays within an enclosed hybridization chamber, comprising:a substrate having a surface with at least a portion of said surface representing a hybridization region, having an area of about 4 mm.sup.2 to about 500 mm.sup.2 wherein the substrate surface is functionalized with a mixture of a first silane providing surface --Si--R.sup.1 groups where R.sup.1 is a chemically inert moiety and a second silane providing surface --Si--(L).sub.n --R.sup.2 where L is a linking group, n is 0 or 1, and R.sup.2 is a functional group enabling binding of the oligonucleotide probes;a plurality of oligonucleotide probes bound to the substrate surface within the hybridization region and arranged in a spatially defined and physically addressable manner;a cover having a peripheral lip which sealingly contacts the substrate surface about the hybridization region, wherein the cover and the hybridization region form an enclosure having an interior space comprising a hybridization chamber having a height of about 50 .mu.m to about 500 .mu.m; and,containing within the hybridization chamber, a sample fluid comprising a target molecule which may hybridize to a surface-bound molecular probe within the hybridization region, and wherein the sample fluid additionally comprises a surfactant of a type and present at a concentration effective to substantially reduce non-specific binding and promote mixing of components within the sample fluid.