초록 ▼

A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component is harvested simultaneously from the clotting and adhesive proteins component from the same donor plasma in less than one hour. The combined components provide an improved biological

A sterile method for preparing stable thrombin component from a single donor's plasma in which the thrombin component is harvested simultaneously from the clotting and adhesive proteins component from the same donor plasma in less than one hour. The combined components provide an improved biological hemostatic agent and tissue sealant by virtue of its freedom from the risk of contaminating viruses or bacteria from allogenic human or bovine blood sources. The thrombin provides polymerization of the clotting and adhesive proteins in less than five seconds, and is sufficiently stable to provide that fast clotting over a six hour period. Further, the clotting times can be predictably lengthened by diluting the thrombin with saline.

대표청구항 ▼

[ I claim:] [1.]1. A manifold for preparing thrombin from plasma, comprising:a coupling on said manifold allowing plasma to enter said manifold;a plasma syringe:a dock for said plasma syringe on said manifold;a receptacle for a solution of CaCl.sub.2 and ETOH;a dock on said manifold to receive said

[ I claim:] [1.]1. A manifold for preparing thrombin from plasma, comprising:a coupling on said manifold allowing plasma to enter said manifold;a plasma syringe:a dock for said plasma syringe on said manifold;a receptacle for a solution of CaCl.sub.2 and ETOH;a dock on said manifold to receive said receptacle;a thrombin receiving syringe;a dock on said manifold for said thrombin receiving syringe to receive the thrombin;a filter located on the manifold and just upstream the thrombin receiving syringe to remove particulate matter;and valve means on said manifold to allow access first between said coupling and said plasma syringe, second between said receptacle and said plasma syringe and third between said plasma syringe and said thrombin receiving syringe.