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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0413565 (1999-10-06) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 651 인용 특허 : 254 |
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agen
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
[ We claim:] [27.]27. A method of monitoring an analyte an electrochemical sensor, the method comprising:contacting a sample with an aperture defined between two substrates of an electrochemnical scraper comprising at least one working electrode disposed on a one of the two substrates;drawing at lea
[ We claim:] [27.]27. A method of monitoring an analyte an electrochemical sensor, the method comprising:contacting a sample with an aperture defined between two substrates of an electrochemnical scraper comprising at least one working electrode disposed on a one of the two substrates;drawing at least a portion of the sample through the aperture and into a sample chamber disposed between the two substrates;observing an indicator electrode disposed on a one of the two substrates, wherein the indicator electrode is positioned within the sensor to indicate, when the sample contacts the working electrode and then subsequently contacts the indicator electrode, that the sample chamber is sufficiently full for monitoring the analyte using the sensor; andgenerating an analyte-responsive signal using the at least one working electrode and at least one counter electrode disposed on a one of the two substrates, wherein the at least one working electrode and at least one counter electrode are separate from the indicator electrode and are disposed in electrolytic contact with the sample chamber.
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