초록 ▼

Oligonucleotides and other macromolecules are provided that have increased nuclease resistance, substituent groups for increasing binding affinity to complementary strand, and sub-sequences of 2'-deoxy-erythro-pentofuranosyl nucleotides that activate RNase H enzyme. Such oligonucleotides and macromo

Oligonucleotides and other macromolecules are provided that have increased nuclease resistance, substituent groups for increasing binding affinity to complementary strand, and sub-sequences of 2'-deoxy-erythro-pentofuranosyl nucleotides that activate RNase H enzyme. Such oligonucleotides and macromolecules are useful for diagnostics and other research purposes, for modulating protein in organisms, and for the diagnosis, detection and treatment of other conditions susceptible to antisense therapeutics.

대표청구항 ▼

[ What is claimed is:] [1.]1. A method of concurrently enhancing hybridization and RNASE H activation in a cell comprising contacting the cell with an oligonucleotide having a sequence of nucleotides which specifically hybridize to a complementary strand of nucleic acid and where at least one of the

[ What is claimed is:] [1.]1. A method of concurrently enhancing hybridization and RNASE H activation in a cell comprising contacting the cell with an oligonucleotide having a sequence of nucleotides which specifically hybridize to a complementary strand of nucleic acid and where at least one of the nucleotides is functionalized to increase nuclease resistance of the oligonucleotide, where a plurality of the nucleotides have a substituent group located thereon to increase binding affinity of the oligonucleotide to a complementary strand of nucleic acid, and where a plurality of the nucleotides have 2'-deoxy-erythro-pentofuranosyl sugar moieties.