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Kafe 바로가기국가/구분 | United States(US) Patent 등록 |
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국제특허분류(IPC7판) |
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출원번호 | US-0295962 (1999-04-21) |
발명자 / 주소 |
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출원인 / 주소 |
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대리인 / 주소 |
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인용정보 | 피인용 횟수 : 711 인용 특허 : 251 |
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agen
A sensor utilizing a non-leachable or diffusible redox mediator is described. The sensor includes a sample chamber to hold a sample in electrolytic contact with a working electrode, and in at least some instances, the sensor also contains a non-leachable or a diffusible second electron transfer agent. The sensor and/or the methods used produce a sensor signal in response to the analyte that can be distinguished from a background signal caused by the mediator. The invention can be used to determine the concentration of a biomolecule, such as glucose or lactate, in a biological fluid, such as blood or serum, using techniques such as coulometry, amperometry, and potentiometry. An enzyme capable of catalyzing the electrooxidation or electroreduction of the biomolecule is typically provided as a second electron transfer agent.
1. A sensor for determining the concentration of an analyte in a sample fluid, the sensor comprising:an electrode pair comprising a working electrode and a counter electrode, wherein at least a portion of the working electrode is within a distance of no more than 200 m of a portion of the counter el
1. A sensor for determining the concentration of an analyte in a sample fluid, the sensor comprising:an electrode pair comprising a working electrode and a counter electrode, wherein at least a portion of the working electrode is within a distance of no more than 200 m of a portion of the counter electrode; a measurement zone positioned adjacent to the working electrode and counter electrode, wherein the measurement zone is sized to contain a volume of no more than about 1 .mu.L of sample; a reference electrode disposed in contact with the measurement zone; and an analyte-responsive enzyme and a diffusible redox mediator disposed in the measurement zone; wherein the sensor is configured and arranged so that the redox mediator oxidizes the analyte and the half-wave potential of the redox mediator, as measured by cyclic voltammetry in 0.1 M NaCl at pH 7, is no more than about +100 millivolts relative to the potential of the reference electrode.
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