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Methods for analysis and sorting of polynucleotides 원문보기

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • G01N-001/18
출원번호 US-0499943 (2000-02-08)
발명자 / 주소
  • Stephen Quake
  • Wayne D. Volkmuth
출원인 / 주소
  • California Institute of Technology
대리인 / 주소
    Darby & Darby
인용정보 피인용 횟수 : 133  인용 특허 : 48

초록 ▼

The invention relates to a microfabricated device and methods of using the device for analyzing and sorting individual polynucleotides, e.g., by size according to an optical signal measured within a detection region of the device. An optical signal such as fluorescence from a reporter molecule assoc

대표청구항 ▼

1. A method of isolating polynucleotide molecules based on size, which method comprises:(a) flowing a stream of a solution containing reporter-labeled polynucleotide molecules through an analysis unit, wherein the analysis unit is microfabricated onto the substrate of a chip and comprises a main cha

이 특허에 인용된 특허 (48)

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  8. Hillman Robert S. (Cupertino CA) Cobb Michael E. (Sunnyvale CA) Allen Jimmy D. (Los Altos CA) Gibbons Ian (Menlo Park CA) Ostoich Vladimir E. (San Jose CA) Winfrey Laura J. (Belmont CA), Capillary flow device.
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  23. Wilding Peter (Paoli PA) Kricka Larry J. (Berwyn PA), Mesoscale polynucleotide amplification device and method.
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  28. Parce John Wallace ; Kopf-Sill Anne R., Methods and apparatus for in situ concentration and/or dilution of materials in microfluidic systems.
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  29. Wilding Peter (Paoli PA) Kricka Larry J. (Berwyn PA) Zemel Jay N. (Jenkintown PA), Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems.
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  45. Kaltenbach Patrick (Bischweier DEX) Swedberg Sally A. (Los Altos CA) Witt Klaus E. (Keltern DEX) Bek Fritz (Waldbronn DEX) Mittelstadt Laurie S. (Belmont CA), Use of temperature control devices in miniaturized planar column devices and miniaturized total analysis systems.
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