초록

The present invention relates in part to methods for screening for novel enzymatic pathways in environmental samples using metabolic selection strategies, and the isolation of the genes and proteins that make up these pathways.

대표청구항 ▼

1. A method of screening for a nucleic acid molecule encoding a metabolic pathway that converts a source compound to a target compound, said method comprising:(1) providing a cell containing one or more genes responsible for converting a target compound to provide a detectable signal, wherein said o

1. A method of screening for a nucleic acid molecule encoding a metabolic pathway that converts a source compound to a target compound, said method comprising:(1) providing a cell containing one or more genes responsible for converting a target compound to provide a detectable signal, wherein said one or more genes are under the control of an inducible promoter, and wherein said detectable signal is not produced in the presence of said source compound; (2) expressing an isolated nucleic acid molecule potentially encoding said metabolic pathway in said cell; and (3) identifying cell that produces a said detectable signal in the presence of said source compound and an inducer of said promoter, but not in the presence of said source compound and absence of said inducer, wherein the identified cell contains a nucleic acid molecule encoding a metabolic pathway that converts said source compound to said target compound.