Dry powder cells and cell culture reagents and methods of production thereof
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-005/00
C12N-001/20
C12N-001/14
C12N-001/16
C12N-001/18
출원번호
US-0023790
(1998-02-13)
발명자
/ 주소
Richard Fike
William Whitford
William Biddle
출원인 / 주소
Invitrogen Corporation
대리인 / 주소
Sterne, Kessler, Goldstein & Fox P.L.L.C.
인용정보
피인용 횟수 :
38인용 특허 :
21
초록▼
The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including po
The present invention relates generally to nutritive medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powder nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine serum (FBS)), medium subgroup formulations and cell culture media comprising all of the necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides powder buffer formulations that produce particular ionic and pH conditions upon reconstitution with a solvent. The invention is particularly directed to methods of production of these media, media supplement, media subgroup and buffer formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells, particularly bacterial cells, yeast cells, plant cells and animal cells (including human cells) using these dry powder nutritive media, media supplement, media subgroup and buffer formulations. The invention also relates to methods of producing sterile powdered media, media supplement (particularly powdered sera such as powdered FBS, powdered transferrin, powdered insulin, powdered organ extracts (such as bovine brain or pituitary extracts), powdered growth factors (such as EGF, FGF, etc.) and the like), media subgroup and buffer formulations. In a particularly preferred aspect, the invention relates to such methods wherein the sterilization is accomplished by gamma irradiation. The invention also relates to methods for producing dry cell powders, comprising spray-drying a cell suspension. The invention also relates to cell, media, media supplement, media subgroup and buffer powders produced by these methods.
대표청구항▼
1. A method of producing an agglomerated eukaryotic cell culture medium powder, said method comprising agglomerating a dry powder eukaryotic cell culture medium with a solvent by:(a) placing said dry powder eukaryotic cell culture medium into a fluid bed apparatus; (b) introducing said solvent into
1. A method of producing an agglomerated eukaryotic cell culture medium powder, said method comprising agglomerating a dry powder eukaryotic cell culture medium with a solvent by:(a) placing said dry powder eukaryotic cell culture medium into a fluid bed apparatus; (b) introducing said solvent into said dry powder under conditions whereby said powder is moistened; and (c) drying said moistened powder, thereby producing an agglomerated eukaryotic cell culture medium powder, wherein said agglomerated eukaryotic cell culture medium powder exhibits reduced dusting and a larger particle size than does said dry powder eukaryotic cell culture medium.
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이 특허에 인용된 특허 (21)
Jensen Jens P. (Hudson WI), Apparatus for crystallizing whey.
Christensen Mogens (Virum DKX) Madsen Benny (Horsens DKX) Bonde Mikael (Basel CHX), Bed plate for a fluidized bed apparatus and a method for making the same.
Prestrelski Steven J. (1971 W. Middlefield Rd. ; Unit #5 Mountain View CA 94043) Zhang Mei Z. (444 Via Colinas Thousand Oaks CA 91362), Formulation of a reconstituted protein, and method and kit for the production thereof.
Miekka, Shirley I.; Burgess, Wilson; Drohan, William N.; Griko, Yuri; MacPhee, Martin J.; Mann, David M., Methods for sterilizing biological materials.
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