초록 ▼

A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consists of a sterilisable disposable container which may be partially filled with a suitable sterile biological cell and/or tissue culture medium. The container has means for

A disposable device and method for axenically culturing and harvesting cells and/or tissue in consecutive cycles. The device consists of a sterilisable disposable container which may be partially filled with a suitable sterile biological cell and/or tissue culture medium. The container has means for removing excess air and/or waste gases therefrom, and means for introducing inoculant and/or culture medium and/or additives therein. A reusable harvesting means enables harvesting of at least a portion of the medium containing cells and/or tissue when desired, thereby enabling the device to be used continuously for at least one subsequent consecutive culturing/harvesting cycle. The portion of medium containing cells and/or tissue remaining from a previously harvested cycle may serve as inoculant for a next culture and harvest cycle.

대표청구항 ▼

1. A disposable device for axenically culturing and harvesting at least one of tissue and a quantity of cells in at least one cycle, said device comprising a sterilisable disposable non-rigid container which is at least one of transparent and translucent, said container having a top end and a bottom

1. A disposable device for axenically culturing and harvesting at least one of tissue and a quantity of cells in at least one cycle, said device comprising a sterilisable disposable non-rigid container which is at least one of transparent and translucent, said container having a top end and a bottom end, which container may be at least partially filled with at least one of a suitable sterile biological cell culture medium, a suitable sterile biological tissue culture medium, axenic inoculant, sterile air and required other sterile additives, said container comprising:(i) gas outlet means for removing at least one of excess air and waste gases from said container; (ii) additive inlet means for introducing at least one of said inoculant, said culture medium and said additives into said container; and characterized in further comprising(iii) reusable harvesting means comprising suitable flow control means for enabling harvesting of at least a desired portion of said medium containing at least one of said tissues and quantity of cells, when desired, thereby enabling said device to be used continuously for at least one further consecutive culturing/harvesting cycle, wherein a remainder of said medium containing at least one of said cells and tissue, remaining in said device from a previous harvested cycle, may serve as inoculant for a next culture and harvest cycle, wherein at least one of said culture medium and said required additives are provided.